LOCUS       sequence-92319-e        8374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses eGFP from an about 1350 bp human FOXA2 promoter to select 
            floor plate and ventral midbrain progenitor cells during 
            development.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Aguila JC, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, 
            Gayosso M, Paz Lopez Mato M, Lopez de Maturana R, Hedlund E, Sonntag
            K, Sanchez Pernaute R
  TITLE     Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for 
            Dopamine Neurons From Human Pluripotent Stem Cells.
  JOURNAL   Stem Cells Transl Med. 2014 Jul 14. pii: sctm.2014-0011.
  PUBMED    25024431
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 Jul 14. pii: sctm.2014-0011."
COMMENT     Sequence Label: Lenti.pFOXA2.5'.GFP
FEATURES             Location/Qualifiers
     source          1..8374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2399..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2700..2825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3318..3551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3735..3779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4046..4163
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     gap             4917..4945
                     /estimated_length=29
     regulatory      5297..5306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5303..5305,5306..5308,5309..6022)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5303..5305,5306..5308,5309..6019)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5348..5369)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5609..5628)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5956..5977
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6038..6627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6091..6111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6509..6520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6715..7348
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7420..7541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7457..7476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7511..7530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7560..7695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7727..7746)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7728..7746)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7756..7773)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7756..7772)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7765..7787)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7917..8372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8004..8023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8214..8235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgga
     2401 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2461 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2641 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc
     2701 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     2761 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     2821 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     2881 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     2941 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     3001 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     3061 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     3121 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     3181 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3241 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3301 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3361 atgggcgcag cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     3421 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     3481 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     3541 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     3601 tggaatgcta gttggagtaa taaatctctg gaacagattg gaatcacacg acctggatgg
     3661 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3721 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3781 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3841 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     3901 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     3961 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4021 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
     4081 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     4141 aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgagcaa ggtccagcca
     4201 ggttttcaga accaagaaag agcctccaca tccaaacacc tgcaatatcc ccccactcca
     4261 aatctgggct cacaggctaa cccagaacag aagacaattt ttgaacccaa gagctgctgg
     4321 ggaaataaaa gtatacgatt gctggagttt ctaatttcta ttaagcagtc cctctggaag
     4381 acagagagga cagagacgct cttgaagtca actccatatg ccccatcatt gattcctgga
     4441 ttcttctctc ctcacccctc cctccccacc tcctgccctg tttgttttag ttacgaaatg
     4501 ctgtgggcac ctcggttgtg actgaaaagt aaccttgaaa cacgccggcc tgaatatcag
     4561 aggcaaatct cagcctccca accgtcggcc gctgctagag gggctgcttg cgccaggcgc
     4621 cggccgcccc actgcgggtc cctggcggcc ggtgtctgag gagtcggaga gccgaggcgg
     4681 ccagaccgtg cgccccgcgc ttctcccgag gccgttccgg gtctgaactg taacagggag
     4741 gggcctcgca ggagcagcag cgggcgagtt aaaggtgtgt acgtagtttt tctaaatagg
     4801 acagcgcttt gcaaattggc tgtcatggac ttgctgtttt gacaganaat gagcactgag
     4861 ggcgaanaaa cccggcataa ataaatccgg ctttcntagg gntctgccgg gcactgnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnntcctc ctccccaccc ccccactagc ccccgcgccg
     4981 cgcgcttccc gccgcctcgg ggcagctcgg cactgccaac ctcagccgcc acccggcagc
     5041 ccgtgagcgc ggcggcggcg ggtgctccct accgcggggc gctggcctgg gcctcctccg
     5101 ctcctcgccc ccccccacct cccgcccact tccaactacc gcctccggcc tgcccaggga
     5161 gagagaggga gtggagccca gggagaggga gcgcgagaga gggagggagg aggggacggt
     5221 gctttggctg actttttttt aaaagagggt gggggtgggg ggtgattgct ggtcgtttgt
     5281 tgtggctgta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5341 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5401 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5461 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5521 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5581 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5641 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5701 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5761 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5821 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5881 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5941 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6001 catggacgag ctgtacaagt aaagcggccg cgtcgacaat caacctctgg attacaaaat
     6061 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6121 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6181 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6241 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6301 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6361 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6421 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     6481 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6541 cggcctgctg cccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6601 ggatctccct ttgggccgcc tccccgccnt ggaattcgag ctcggtacct ttaagaccaa
     6661 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6721 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6781 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     6841 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     6901 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6961 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     7021 atccggagta cttcaagaac tgctgacatc gagcttgcta caagggactt tccgctgggg
     7081 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatgctgca
     7141 tataagcagc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7321 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7381 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7441 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7501 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7621 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7681 gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc
     7741 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7801 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7861 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc
     7921 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7981 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8041 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8101 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8161 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8221 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8281 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8341 gaattttaac aaaatattaa cgtttacaat ttcc
//
