LOCUS       sequence-92321-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA SF-HERC2 F2
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..178
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             188..211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac gcgtccacca tggactacaa
      121 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg
      181 gtcggcctgg agccacccgc agttcgaaaa aggatccact agtccagtgt ggtggaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtgcagcc attactgcag agatccagga
      301 tattgaagcc aaaaaagaag cacagaagga aaaagaaatt gatgaacagg aagcgaatgc
      361 ctcaacattt catagaagca ggactccact ggataaagac cttattaata cggggatctg
      421 tgagtcttct ggcaaacagt gtttgcctct ggttcagctc atacaacagc ttcttagaaa
      481 cattgcttct cagactgtag ccagattgaa agatgttgcc cgtcggattt catcatgtct
      541 ggactttgag caacacagtc gtgaaagatc tgcttcattg gatttgttac tgcgttttca
      601 acgtttgctt attagtaaac tttatccagg agaaagtatt ggtcagacct cagatatttc
      661 tagtccagag ctaatgggtg ttggttcctt gctgaagaag tacacagccc tcctgtgcac
      721 gcacattgga gatatactgc ctgtggccgc cagcattgct tctaccagct ggcggcactt
      781 cgcggaggtg gcttacattg tggaagggga ctttactggt gttctccttc cagaactagt
      841 agtttctata gtgcttctgc tcagtaaaaa tgctggtctc atgcaagagg ctggagctgt
      901 acctctgctg ggtggcctgt tggaacatct ggatcggttc aaccatctgg caccaggaaa
      961 ggaacgggat gatcatgaag agttagcctg gcctggcata atggagtcat tttttacagg
     1021 tcagaactgt agaaataatg aggaagtgac acttatacgc aaagctgatt tggagaacca
     1081 taataaagat gg
//
