LOCUS       sequence-92324-e        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA SF-HERC2 F3
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..178
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             188..211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac gcgtccacca tggactacaa
      121 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg
      181 gtcggcctgg agccacccgc agttcgaaaa aggatccact agtccagtgt ggtggaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtattcct gagggaatcg atatagggga
      301 acctcttact gattgtttaa aggatgttga tttgatcccg ccttttaatc ggatgctgct
      361 ggaagtcacc tttggcaagc tgtacgcttg ggctgtacag aacattcgaa atgttttgat
      421 ggatgccagt gccaaattta aagagcttgg tatccagccg gttcccctgc aaaccatcac
      481 caatgagaac ccgtcaggac cgagcctggg gaccatcccg caagcccgct tcctcctggt
      541 gatgctcagc atgctcaccc tgcagcacgg cgcaaacaac ctcgaccttc tgctcaattc
      601 cggcatgctg gccctcacgc agacggcact gcgcctgatt ggccccagtt gtgacaacgt
      661 tgaggaagat atgaatgctt ctgctcaagg tgcttctgcc acagttttgg aagaaacaag
      721 gaaggaaacg gctcctgtgc agctccctgt ttcaggacca gaactggctg ccatgatgaa
      781 gattggaaca agggtcatga gaggtgtgga ctggaaatgg ggcgatcagg atgggcctcc
      841 tccaggccta ggccgcgtga ttggtgagct gggagaggac ggatggataa gagtccagtg
      901 ggacacaggc agcaccaact cctacaggat ggggaaagaa ggaaaatacg acctcaagct
      961 ggcagagctg ccggctgctg cacagccctc agcagaggat tcggacacag aggatgactc
     1021 tgaagccgaa caaactgaaa ggaacattca ccccactgca atgatgttta ccagcactat
     1081 taacttactg cagactcttt gtctgtctgc tggagttcat gctgaa
//
