LOCUS       sequence-92325-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for anti-mouse Smoothened antibody production (for affinity 
            purification).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Rohatgi R, Milenkovic L, Scott MP
  TITLE     Patched1 regulates hedgehog signaling at the primary cilium.
  JOURNAL   Science. 2007 Jul 20;317(5836):372-6.
  PUBMED    17641202
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-07-20"; volume: "317(5836)"; pages: "372-6"
COMMENT     Sequence Label: pET28 mSmo-Cterm
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atcctggcgc gcctcagaag tccgagtctg catccaagat
      121 ctcagcctcc attaggttag tgcgggaatg aatggatccc agcccctgga ggcggccctg
      181 agcccagacc cggggggctg aggcaccagg gagaaatgga tcttgatggg gactaggctc
      241 tgggcagaag gggttggaga ctagagtcca ggctccctgt cggcaggact cccctgttcc
      301 ccaggcgttt gcagctacca ggcacttctg ccgaggcagc tgaggcagcc gaggaactgc
      361 actggtggca ggaacagggg cagagtggtg aagctcaggg gctggcccca aggggcacac
      421 ctccttcttc ctcttcctcc gctttttctt ccggcccaaa cgcttctcta actctgggga
      481 gatctctgcc tcaaccagcc acatgttggc ttgttcttct ggtggcactg gagttgccac
      541 aggggtaacg gacacatcct ggggcaatat ggctcctctc cgagccacca tcttggtgac
      601 atgctgagcc caggcagagg agacgtcagc tgagggttca ttgaggtcaa aagccaaacc
      661 cgcaacaggt ccatcatggg agacagtgtg catgctgaag gagagctcct ggcccgggtt
      721 ctgcagcagc tcacgccgct tagagaaggc cttggcgatc atcttgctct tcttgattct
      781 cttgggctca tcatcactgt gcccagtcaa cctgcaccag gtgcgcctcc agatgagcag
      841 g
//
