LOCUS       sequence-92330-e        2866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector with RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pENTR4-RBPMS
FEATURES             Location/Qualifiers
     source          1..2866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1097..1196)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1308..1327)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1384..1403)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2216..2804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2705..2724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gacatgaaca acggcggcaa agccgagaag gagaacaccc cgagcgaggc caaccttcag
      541 gaggaggagg tccggaccct atttgtcagt ggccttcctc tggatatcaa acctcgggag
      601 ctctatctgc ttttcagacc attaagggct atgagggttc tcttataaag ctcacatcta
      661 aacagcctgt aggttttgtc agttttgaca gtcgctcaga agcagaggct gcaaagaatg
      721 ctttgaatgg catccgcttc gatcctgaaa ttccgcaaac actacgacta gagtttgcta
      781 aggcaaacac gaagatggcc aagaacaaac tcgtagggac tccaaacccc agtactcctc
      841 tgcccaacac tgtacctcag ttcattgcca gagagccata tgagctcaca gtgcctgcac
      901 tttaccccag tagccctgaa gtgtgggccc cgtaccctct gtacccagcg gagttagcgc
      961 ctgctctacc tcctcctgct ttcacctatc ccgcttcact gcatgcccag atgcgctggc
     1021 tccctccctc cgaggctact tctcagggct ggaagtcccg tcagttctgc tgagcggccg
     1081 cactcgagat atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1141 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1201 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1261 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1321 gagccatatt caacgggaaa cgtcgaggcc gcgataaatc caacatggat gctgatttat
     1381 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     1441 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     1501 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     1561 tcaagcattt tatccgtact cctggtgatg catggttact caccactgcg atccccggaa
     1621 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     1681 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     1741 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     1801 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     1861 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     1921 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1981 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     2041 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     2101 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag
     2161 attgggcccc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2341 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2821 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
