LOCUS       sequence-92347-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse codon-optimized PhiC31 recombinase with bovine 
            growth hormone polyadenylation (bpA) sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Raymond CS, Soriano P
  TITLE     High-efficiency FLP and PhiC31 site-specific recombination in 
            mammalian cells.
  JOURNAL   PLoS One. 2007 Jan 17;2(1):e162. doi: 10.1371/journal.pone.0000162.
  PUBMED    17225864
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2007-01-17"; volume: "2(1):e162. doi"; pages: " 
            10.1371/journal.pone.0000162"
COMMENT     Sequence Label: pPGKPhiC31obpA
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        89..588
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(124..147)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     521..540
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      598..607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
      121 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
      181 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
      241 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
      301 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
      361 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
      421 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
      481 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
      541 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac catcgccgcc
      601 accatggata cctacgccgg agcctacgac agacagagcc gggagagaga gaacagcagc
      661 gccgccagcc ccgccaccca gagaagcgcc aacgaggata aggccgccga tctgcagaga
      721 gaggtggaga gggacggcgg cagattcaga tttgtgggcc acttcagcga ggcccctggc
      781 accagcgcct tcggcaccgc cgagagaccc gagttcgaga gaatcctgaa cgagtgtagg
      841 gccggcaggc tgaacatgat catcgtgtac gacgtgtccc ggttca
//
