LOCUS       sequence-92352-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-UbiquitinC-C-18
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..294
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    427..548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(464..483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     518..537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(692..713)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc ggcagcggtg
       61 gagagcggtc agatcttcgt gaagactctg actggtaaga ccatcaccct cgaggttgag
      121 cccagtgaca ccattgagaa tgtcaaggca aagatccaag ataaggaagg catccctcct
      181 gaccagcaga ggctgatctt tgctggaaaa cagctggaag atgggcgcac cctgtctgac
      241 tacaacatcc agaaagagtc caccctgcac ctggtactcc gtctcagagg tgggtgagga
      301 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
      361 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      421 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      541 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      601 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      661 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      721 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      781 tgaaccatca ccctaatcaa gtttt
//
