LOCUS       sequence-92363-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(68..355)
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     promoter        complement(390..593)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(419..439)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
       61 ttcgtcccga acgagtgtac ttgccccaaa tgtgcaacat aaatacagaa gcgatgaaca
      121 gaagactcat aaccaatact ggaacagggc caactttgag cccaggtgaa tcttctgtgt
      181 agaatcgcca catccccccg gtgcctgccg aggttgtgcg gcctgcactc cttgtcccac
      241 agctggcatt tttcctctgc cggacagtgg atcccgccgc ccgggcggcc actgctttgc
      301 tgggagagcg ccctgaggat cccacgttag tgccactggg ggtcggacca ggcatggtgg
      361 cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
      421 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      481 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      541 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      601 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      661 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      721 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
      781 cgtcaatgga aagtccctat tggcgttact atgg
//
