LOCUS       sequence-92379-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Sec61-C-18
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..357
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     polyA_signal    518..639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(555..574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     609..628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(783..804)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gacgagctgt acaagtccgg actcagatct ggctccagcg caggcagcgc atccggcgga
       61 agcggaagcc ctggtccgac ccccagtggc actaacgtgg gatcctcagg gcgctctccc
      121 agcaaagcag tggccgcccg ggcggcggga tccactgtcc ggcagaggaa aaatgccagc
      181 tgtgggacaa ggagtgcagg ccgcacaacc tcggcaggca ccggggggat gtggcgattc
      241 tacacagaag attcacctgg gctcaaagtt ggccctgttc cagtattggt tatgagtctt
      301 ctgttcatcg cttctgtatt tatgttgcac atttggggca agtacactcg ttcgtaggaa
      361 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
      421 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      481 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
      661 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      721 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      781 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      841 a
//
