LOCUS       sequence-9239-ex        5791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5791)
  TITLE     Forth generation Seydoux lab vectors
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCM3.11
FEATURES             Location/Qualifiers
     source          1..5791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3967..4066)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4178..4197)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4189..4998
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4256..4275)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5141..5729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5630..5649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatggaggc taacaagcga gaaattgtgg atttcggtgg gctgagatcc
      481 tactttttcc caaatttggc ccactacatc acgaagaatg acgaagaact gttcaacaat
      541 acatctcagg ttggactata atatttaaag accgattttc tattattcaa tttcaggcca
      601 ataagttggc tgctttcgtg ttgggagctt ccaaagacgc tcctggagac gaagacattt
      661 tggagatgat cttgccgaac gacgctaacg ctgccgttat tgctgctggc atggacgttt
      721 gtcttcttct cggagataaa tttgtaagtg tgaattaaac ttattgtaat caattataac
      781 gttttagcgt ccgaaatttg atgctgctgc tgagaaattg tcaggattgg gacatgctca
      841 tgatctggtt tctgtcatcg atgacgacaa gaagctcgga atgctcgccc gtaaggccaa
      901 gctgaagaag actgaagacg ctaagattct tcaagctctt ctcaaagtaa tcgcaatcga
      961 tgatgccgcc gaaaagtttg ttgagctcac ggaacttgtc agccaactgg atctggactt
     1021 tgatgtttat gtcctaacta aaatcctcgg acttatctct gaggagacta gcgacgaggt
     1081 cgacattatc cgtgataacg tcgtcaacgc atttgatagc tgcaagccgt tgttgaaaca
     1141 gttgatgtaa ttgttgtcct tttcaaaata attcgatttt ttttttcaat ttaggttgga
     1201 tggtccgaaa tctgaacctg ccgatccatt cattagtctc ctcatggatc cactcgaaga
     1261 gtctgttggg aaagttgtga accatatcgc tcaacttttc gaggaagcga gtaaaaatga
     1321 aggagacgag tcacttgtct tgagatctca actcggatat cagctctttt tccttatcgt
     1381 tcgttcactt gcagatggaa agcgcgaggt ttccaagaaa atcctctctg ggattcctac
     1441 gagtgtcaga gcggaagtct ttccaggatt gcaaagaagt gtctacaaga gtgcggtgtt
     1501 cctcggcaat cacatcatcc aagttctttt aggatgttag tattttcgtc tttgtttact
     1561 tttaaaatct aaatatttca gctaagaagt cttttgagga ttgggatgtt gttggagtcg
     1621 cgaaagactt ggagagcgcc tggaaaagac gagccattgc ggaacttatc aagaagttcc
     1681 aagtctcaat tctagagcaa tgcttcgata agcccgttcc actggtgagt taatgattct
     1741 ataaacatta cttacatgac cgtttttcag attcctcaga gtccactaaa caatgatgcg
     1801 gtgatcgata atgtgaataa agctctacag tttgcactgt ggcttacaga gttttacgga
     1861 tccgagaatg agactgaagc gctgggcgag cttcgtttct tggattccac ttcaaaaaat
     1921 cttctcgtcg atagcttcaa gagtgaatat tccttttttt atctcctgtt aactttttaa
     1981 tttcagaatt tgttcaagga atcaactcga agactcatgt cactcgaatt gttgaatcac
     2041 tggagaagtg ttgtttgtcc gatacaccaa gtggcagaaa gagcaatgtg caaccatcca
     2101 cttcccaaca gcaggattcc gcctacacga aagaggaaat gacgacggtt cacaacacat
     2161 acagcgtaaa tactaaagct caagttctca acggtctcag tgataccaat tcttccgggt
     2221 tacttgtcga ttcaaaggat tcactcagtc ttcaggagat ttcttgtgac gaagtggatt
     2281 cttcgacttt gctaagctca tcaagaaaca ttggagaagg tgttactgta aaagcggtcg
     2341 atcctgtccc agaaaaggtt aacgatgcac aacaacagca aacagttaac gagatcgaga
     2401 tggcttcgga tgccaatcaa gatacttctt caagtgcttc gccagaagta gcgccaagtt
     2461 tttccaccga tggctgggat tctccaacga aatcagtagc tctgccaccg gtatcgcgtt
     2521 catcgtttca gaattataaa aatatttctt tcagggcatg cagcagatag atgaagagga
     2581 aaccacagtt gctgacaaag attctacacc acaaccacaa gctcgtgctg aaactgcatg
     2641 ggggtcaggc gacgctactc caatgcctct tcctgccccg actaatcaat acaaagtcag
     2701 tggatttggg gaagccaaag ttgcaaaagg attcggtcaa tttgctccca ccagttcagc
     2761 ttatggagga ggaggtggtc gcggaggata tggcggtgga gaccgtggag gcagaggtgg
     2821 atacggcgga gatcgtggag gtagaggtgg atacggcggg ggagaccgcg gaggacgagg
     2881 tggatacggc ggagatcgtg gacgtggtgg ttacgggggt cgtggtggac gcggaggttt
     2941 ctaaactcca actattgaat gtttaatttg ttttttaagt atacacttct tgtatactta
     3001 cacgttttac gcacattatt tattctaaca atcttgttgt aaaatcccta aaaccttgac
     3061 tgtcatttat tatttattca acctgttttt ccttgttccc ggtaaatgtt tgaactagat
     3121 tgttgcaaga aattaactca attaattaca cgtatatctc tttctgcggc ttgatgagaa
     3181 aagtgatgtc tgaatcgacg gtgccgaatg attggtggat cataaaatta acaggtggca
     3241 ctgcatgcat tcttatacaa tttcaactga tcttatcata catttacgca tattagagtt
     3301 gtaaatcgtt atgagacatc tgcattcgaa cataacaaat accgtatttc ttctattaat
     3361 atggctgcaa tactattttt cgatggtctt cccgcttgca atactaatag ggaatgcaag
     3421 actattaggg agtgcaatac taattttcag agcattttct gactttgagc ttactagttt
     3481 tttcctgaaa aagctcgaat catatgtaaa aattcagaaa atttgatttt catataagtt
     3541 caaaaacttc gatctcgtag aaatgatgtg aaaactgttg caaaataggc aaaaaaatgt
     3601 tgaaatccta aaattagcga gacgggtttg caatacaaaa aagttaacgc aagactacta
     3661 gagagtgcaa gactaatagg gtgtgcaata ctaattttcg gaacgtctcc gaggggcaat
     3721 actaataggg agtgcaagtc taatagggag gccatattaa tagaagatat acggtaatat
     3781 acattttcat aagataatac aaattgtaat aaaacaatca gttcacacag tgtagatcat
     3841 ttggaaatcg ttttctaatc ttaaaatcaa ttgattcgca gaaaacagga tgttgtttat
     3901 aaataaattc acctcgcttc acaaccttct cttccagtac tttcgaaaat tcagagtcca
     3961 tttgacaccc agctttcttg tacaaagtgg gcattataag aaagcattgc ttatcaattt
     4021 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag
     4081 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     4141 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     4201 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     4261 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     4321 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     4381 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     4441 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     4501 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     4561 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     4621 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     4681 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     4741 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     4801 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     4861 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     4921 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     4981 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     5041 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5281 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5521 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5761 tggccttttg ctggcctttt gctcacatgt t
//
