LOCUS       sequence-92393-e        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA SF-HERC2 F5
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..178
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             188..211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac gcgtccacca tggactacaa
      121 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg
      181 gtcggcctgg agccacccgc agttcgaaaa aggatccact agtccagtgt ggtggaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtgtagac ctgctcaagc tgtcagtgtg
      301 cagccgggcc ggggacaggg gcagggatgt gctctccgcg gtgctttccg gcatggggac
      361 cgcctaccca caggtggcag atatgctgtt ggagctctgt gtcaccgagt tggaggatgt
      421 ggccacagac tcgcagagcg gccgcctctc ttctcagcct gtggtggtgg agagtagcca
      481 cccttacacc gacgacacct ccaccagtgg cacagtgaag ataccaggtg cagaaggact
      541 cagggtagaa tttgaccggc agtgctccac agagaggcgc cacgaccctc tcacagtcat
      601 ggacggcgtc aacaggatcg tctccgtgcg gtcaggccga gagtggtccg actggtccag
      661 cgagctgcgc atcccagggg atgagttaaa gtggaagttc atcagcgatg ggtctgtgaa
      721 tggctggggc tggcgcttca ccgtctatcc catcatgcca gctgctggcc ctaaagaact
      781 cctctctgac cgctgcgtcc tctcctgtcc atccatggac ttggtgacgt gtctgttaga
      841 cttccgactc aaccttgcct ctaacagaag catcgtccct cgccttgcgg cctcgctggc
      901 agcttgtgca cagctgagtg ccctagctgc cagtcacaga atgtgggccc ttcagagact
      961 gaggaagctg cttacaactg aatttgggca gtcaattaac ataaataggc tgcttggaga
     1021 aaatgatggg gaaacaagag ctttgagttt tacaggtagt gctcttgctg ctttggtgaa
     1081 aggtcttcca gaagctttgc aaaggcagtt tgaatatgaa gatc
//
