LOCUS       sequence-9240-ex        4314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4314)
  TITLE     Forth generation Seydoux lab vectors
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCM3.12
FEATURES             Location/Qualifiers
     source          1..4314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2490..2589)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2701..2720)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2712..3521
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2779..2798)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3664..4252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4153..4172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgtccga cgaagaaatt gacatcagta ctgctctcaa caacaagact
      481 acaccaaaga agaaatcggt aagcaaagat cagaagtagt cgtctcggca gaggttgaca
      541 tcatttctga caatggatat gacaacttag tcaactttgt tggagaagat cctattgctt
      601 ggccgatatc tcacttatga ttgattatag catgaacgaa acctcatatt cggaaaagta
      661 ttaaatttgg tgccgcctac ctgattgcct cgctcctgct tcctgataca atccgccatc
      721 aacacgattg caaattacat tgaaacattt tttcagctca aaagaaactc caactcgcaa
      781 gaaggttatg aaagtccaga ggaacgtgag attgtctatc catcagtatt tggagccatt
      841 ggaacaccga tggccaaatc ggataacgca aaagaatggg acgaatggaa agaaaaagaa
      901 cgaaaaaaag ataaagctga atggaaacga tatttgagat ccaagtggga tatgactcaa
      961 ggccacttgc cgttggtttc cgattcagag tttttgaaag gaagaaagga gcacaaagag
     1021 tacaattcaa aagctcgcat ggacagtgag ttaataatta aatagttaat ggaaatttta
     1081 aaccattttc agtacttgat ggcctggatg aggttaatga gggattcttc aactgcggga
     1141 aaggagcggc tatgaatatt cgttataatg ataaaaatgt ttcaaagaag ggagcaaaga
     1201 agtttgtagc tactgttggt aagtgtaatc tctggtgaat aacgtcaaaa gtgcaattgc
     1261 tagtaagaca aaactaacgt taaatggagg atcggatttg tggatctgct aaattcgatg
     1321 ccataacaaa aaatgtctag aagagtccta aaccgaattt ccccagtgaa tatgcaaaaa
     1381 aatttttcag aaaccgcaat gaagaaagca ggtaacccga caatggagca gatgatgacg
     1441 gatgatttgg atgaagatga agctcgtgct gaagccgaat gggaacgaca acgtgaacag
     1501 agaaagttag cttcaagagc ttatgatgct gcgatggatg agcgtgtatg atatactatc
     1561 tacaaatttc catttaaatt tcaaaattac aggaggacga tgcaaagtat gtaccttggg
     1621 acgagtactg tcaagagatg gaggagcttg gaaaagaatt gaagattgga gaaaagcatt
     1681 acaaaaaggt ttaactttga aatactatac actgttgtaa tgttttatta tagtggcttg
     1741 aaaagaagat ggatgagaat aaggttactc acaaattcaa tgcatatcag ttggatctca
     1801 agtgtctcga tgaggacgct ttttccaata agtgagtttg gagaaatgat gaaaatgttt
     1861 aacattaaaa atttcagaaa atcattgaaa agtgttgtga gaaacgttca aaagttttat
     1921 agaaagatgc gtgagccgaa gaagtgactc gtgatgatta gcttactcgt gaaatattga
     1981 actatagaaa gagcaataaa gcttattcga atacttacaa tattattcta aaccaaatgc
     2041 atttcaacaa tcaatatttc aatatacata taaagtcacc tttttttcta tctactcctc
     2101 aatttttatt gttgatcgat atcgagtgca aattcagaat taactagaga tttgaaacag
     2161 tgataatgtg caacaagttt gccatcgatc acacgacgcc ttttaggcac aacgtagact
     2221 ggccggctct gtacgagcat gttgcacata aattcatccc aaatttgatt cctattccga
     2281 aaatgcgctt caatttggtc ctagccgggc gttctgaggt tctttggagc ttttttttca
     2341 tttcgaatct ttcaaaatgt tttaaaccaa gagttgaatc ctttattata tcacagaaaa
     2401 aacttaaaaa aaaaggaaca aaacaaaaac attgagattt gtacaatacc cataataaaa
     2461 tatccctaaa aagtgaaaag agaaacaaga cccagctttc ttgtacaaag tgggcattat
     2521 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2581 cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2641 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2701 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2761 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2821 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2881 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2941 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     3001 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     3061 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     3121 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     3181 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     3241 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3301 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3361 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3421 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3481 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3541 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3781 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
