LOCUS       sequence-92400-e        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA SF-HERC2 F6
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..178
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             188..211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac gcgtccacca tggactacaa
      121 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg
      181 gtcggcctgg agccacccgc agttcgaaaa aggatccact agtccagtgt ggtggaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtaaccgc atccaggtca aacgatcaag
      301 gagcaaaggc gggctggccg gccccgacgg caccaagtct gtctttgggc agatgtgtgc
      361 taagatgagc tcgtttggtc ccgacagcct cctccttcct caccgtgtct ggaaagtcaa
      421 gtttgtgggt gaatctgtgg atgactgtgg gggcggctac agcgagtcca tagctgagat
      481 ctgtgaggag ctgcagaacg gactcacgcc cctgctgatc gtgacaccca acgggaggga
      541 tgagtctggg gccaaccgag actgctacct gctcagcccg gccgccagag cacccgtgca
      601 cagcagcatg ttccgcttcc tgggtgtgtt gctgggcatt gccatccgaa ccgggagtcc
      661 cctgagcctc aaccttgccg agcctgtctg gaagcagctg gctgggatga gcctcaccat
      721 cgcggacctc agtgaggttg ataaggattt tattcctgga ctcatgtaca tccgagacaa
      781 tgaagccacc tcagaggagt ttgaagccat gagcctgccc ttcacagtgc caagtgccag
      841 tggccaggac attcagttga gctccaagca cacacacatc accctggaca accgcgcgga
      901 gtacgtgcgg ctggcgataa actatagact ccatgaattt gatgagcagg tggctgctgt
      961 tcgggaagga atggcccgcg ttgtgcctgt tcccctcctc tctctgttca ccggctacga
     1021 actggagacg atggtgtgtg gcagccctga catcccgctg caccttctca agtcggtggc
     1081 cacctataaa ggcatcgagc cttccgcatc gct
//
