LOCUS       sequence-92402-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses halo tagged PTEN protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Fang X, Fu Y, Long MJ, Haegele JA, Ge EJ, Parvez S, Aye Y
  TITLE     Temporally controlled targeting of 4-hydroxynonenal to specific 
            proteins in living cells.
  JOURNAL   J Am Chem Soc. 2013 Oct 2;135(39):14496-9. doi: 10.1021/ja405400k. 
            Epub 2013 Sep 18.
  PUBMED    24015839
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja405400k";
            journalName: "J Am Chem Soc"; date: "2013-10-2- 2"; volume: "135"; 
            issue: "39"; pages: "14496-9"
COMMENT     Sequence Label: pMIR-His-Halo-Tev-PTEN
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             659..676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             686..703
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agagaattac cctcactaaa gggaggagaa gcatgaattc aaggtaccag atcttagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtgga tccaccatgg gcagcagcca
      661 tcatcatcat catcacagca gcggcctggt gcctcgtggt agccatatgg cagaaatcgg
      721 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
      781 cgatgttggt ccgcgcgatg gcaccc
//
