LOCUS       sequence-92403-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-UbiquitinC-N-18
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(68..295)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     promoter        complement(330..533)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(359..379)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        534..837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
       61 ttccaccccc acctctgaga cggagtacca ggtgcagggt ggactctttc tggatgttgt
      121 agtcagacag ggtgcgccca tcttccagct gttttccagc aaagatcagc ctctgctggt
      181 caggagggat gccttcctta tcttggatct ttgccttgac attctcaatg gtgtcactgg
      241 gctcaacctc gagggtgatg gtcttaccag tcagagtctt cacgaagatc tgcatggtgg
      301 cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
      361 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      421 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      481 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      541 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      601 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      661 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
      721 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
      781 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
      841 aactccatat atgggctatg aactaatgac c
//
