LOCUS       sequence-92404-e        2781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI Intron c1
FEATURES             Location/Qualifiers
     source          1..2781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     270..288
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(326..348)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     448..467
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     661..683
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     675..692
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     676..692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1109..1125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1109..1125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1122..1144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1133..1149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1157..1163,1164..1181,1182..1187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1202..1223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1340..1357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1511..2099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1591..1610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2214..2219,2220..2236,2237..2242)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             join(2431..2781,1..183)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
       61 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
      121 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
      181 taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca
      241 caacgccgct catgacatta acctataaaa ataggcgtat cacgaggccc ttttgtctcg
      301 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag aaggtcacag
      361 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      421 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      481 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
      541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      661 cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtacc tcgtaggtct
      721 ctaaggagct tctcctcctc tgctaacgta agcctctctg ttttttttct ctgtttcttt
      781 tgaaatgaat ccaattagtg atgataatct gtgtttgatg tatcattgat ttaacatctt
      841 gacaatgaat cgtgatcgga agtgataaag ttatgggtca acggtttcaa agagagagaa
      901 agacttttag agtcaactct cgactctttc ttaattatgt tattgctatt tgtctctttt
      961 cttgaagtct gaacaattct tgggattgtt ttgcaggttc tagcttctcc aaccacagga
     1021 attcctttga gaccatcgaa tgcatctaga tatcggatcc cgggcccgtc gactgcagag
     1081 gcctgcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2161 ggattttggt catgaggcgc acaccgtgga aacggatgaa ggcacgaacc cagttgacat
     2221 aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2281 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2341 actgtttttt tgtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2401 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagcaacga tgttacgcag
     2461 cagggcagtc gccctaaaac aaagttaggt ggctcaagta tgggcatcat tcgcacatgt
     2521 aggctcggcc ctgaccaagt caaatccatg cgggctgctc ttgatctttt cggtcgtgag
     2581 ttcggcgacg tagccaccta ctcccaacat cagccggact ccgattacct cgggaacttg
     2641 ctccgtagta agacattcat cgcgcttgct gccttcgacc aagaagcggt tgttggcgct
     2701 ctcgcggctt acgttctgcc caggtttgag cagccgcgta gtgagatcta tatctatgat
     2761 ctcgcagtct ccggcgagca c
//
