LOCUS       sequence-92408-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of AP1S3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Setta-Kaffetzi N, Simpson MA, Navarini AA, Patel VM, Lu HC, Allen 
            MH, Duckworth M, Bachelez H, Burden AD, Choon SE, Griffiths CE, 
            Kirby B, Kolios A, Seyger MM, Prins C, Smahi A, Trembath RC, 
            Fraternali F, Smith CH, Barker JN, Capon F
  TITLE     AP1S3 mutations are associated with pustular psoriasis and impaired 
            Toll-like receptor 3 trafficking.
  JOURNAL   Am J Hum Genet. 2014 May 1;94(5):790-7. doi: 
            10.1016/j.ajhg.2014.04.005.
  PUBMED    24791904
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ajhg.2014.04"; journalName: "Am J Hum Genet"; date: 
            "2014-05-1- 1"; volume: "94"; issue: "5"; pages: "790-7"
COMMENT     Sequence Label: pcDNA3.1-myc-AP1S3
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             640..669
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             685..702
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(746..763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    752..863
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagact
      121 cgagcggccg ccactgtgct ggatatctgc agaattccga tgatacattt catattgctc
      181 ttcagtcgac aagggaaatt acggctacag aaatggtaca tcactctccc tgataaagag
      241 aggaagaaga tcacccggga aattgttcag attattctct cccgtggtca caggacaagc
      301 agttttgttg actggaagga gctaaaactt gtttataaaa ggtatgctag tttatatttt
      361 tgctgtgcaa tagaaaatca ggacaatgag ctcttgacgc tagagattgt gcatcgttac
      421 gtggagctgc tggacaaata ttttggaaat gtctgtgagc tggatattat ctttaatttt
      481 gaaaaggctt atttcatcct ggacgagttt ataataggtg gggaaattca ggaaacatcc
      541 aagaaaattg ctgtcaaagc cattgaagac tctgatatgt tacaggagac aatggaagaa
      601 tacatgaaca agcctacatt tggtaccaag cttgggcccg aacaaaaact catctcagaa
      661 gaggatctga atagcgccgt cgaccatcat catcatcatc attgagttta aacggtctcc
      721 agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
      781 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
      841 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
      901 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcagg
//
