LOCUS       sequence-92409-e        2814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of Patronin CC truncation (using 
            Baculodirect kit from Invitrogen).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pENTR-GFP-Patronin CC 776–1457
FEATURES             Location/Qualifiers
     source          1..2814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    727..751
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagcac
      721 cggtatacaa gtttgtacaa aaaagcaggc tccgcggccg cccccatgaa cgccccagtc
      781 agcgcataca gctcccgtcc gcccagtcgc gatccctacc agcagcagct ccaccatcag
      841 cagcagcagc ccatgcccat gccgcaaccg atgcagtacg tcaacgagca tgggcagtat
      901 atgtcgccgc cgcagcccgc acactacatg ccgcagcagg cacaacagcc gcagagcatt
      961 tacagcgaca acggggcggc gtacaatcac agtaaccact cgccatacgg cggagctcca
     1021 cagtatcgga gcagcgtggt gtacgacgat tacgggcagc ccaccaacca cttctacctg
     1081 catgagtcat cgccgcagcc tcaagctcat cagcatccgc agcgcaggac ttgggctcac
     1141 tccgcagcag ccgccgctta tgaacaacag caacagatcc agccttccct ggtggatgtg
     1201 aatgcctggc agacgcagca gcaccaaaag cagaagcaga cctggatgaa caggccgccc
     1261 tcgagtgcgg gagccccgag tcctggcagc tttatgctgc accagaacgg tggaggagga
     1321 ggtggtggcg gtggtggtgg cgagctacag cacctgtttc aggtacaggc ctcgccacag
     1381 catggccaac ggcaggttag tggatccaat ggcgtgcagc gccagcagtc gttgaccaac
     1441 ttgcgagaca atcgctcgcc caaggcacca caaaacatgg gaatgcccat gggtatgcca
     1501 atgcagcaag aggacatgat ggcaccgcag agtatttgct ttatcggtga cgaggaggat
     1561 gttgatgagc tggagcggaa catcatcgaa tctatgcagt cgacgcacat ctccgacttt
     1621 gtacaccaac agcagcagca gcaccaacac caacagcaac ttcagcagca acaacggttg
     1681 caggggcaca gcggacgagg tagcagctcg gaggattatg acagcgggga gatgatctcc
     1741 aacaagctga acatcaccag cggcaatctc acctatcgca taccctcgcc atcccgtccc
     1801 tccatccaag ccaacagctt ccaggatccc cgagccatgg cagctgcttc cggtggcgag
     1861 gaccagccgc ccgagaaggg tttctacatc tccttcgacg atgagcagcc caaacgaccc
     1921 aagccacctc tgcgcgccaa gcgatcgcct aaaaaggagt ctccaccagg aagtcgggac
     1981 agcgtcgata accaggcgac cctgaaacgt gaatcgctaa gtcatctgca caacaacaac
     2041 aatattggat tcggaaatga tgatgtcaac agcaaaccgg tgaccaggca cagtatccat
     2101 ggcctaaaca actccaacag tgtcaaatct cccggaaatg ccacgtacaa caagtacacg
     2161 gatgagccgc ccatccaact gcgacagttg gccgtttcgg gagcaatgtc accaactagt
     2221 aacgaacgtc atcacttgga cgatgtcagc aatcagtcac cgcagcagac gcagcaacca
     2281 atgtcgccca cgcgactcca acagagcagc aacaacgcag aggcggccaa gaacaaggcg
     2341 ctggtcatcg gagcagattc caccaatttg gatccggaat ctgttgatga gatggagcgg
     2401 cgcaaggaga aaatcatgct gctgtctttg caacgtcgcc agcaacagga ggaggcgaag
     2461 gcgcgcaaag agattgaggc ttcccagaag cgagaaaagg agcgcgaaaa ggaggaggaa
     2521 cgagcgcgga aaaaggagga gcaaatggca cggcgagcgg ccattttgga gcagcacaga
     2581 ctcaagaaag ccattgaaga ggccgagcga gagggtaaaa ccctggatcg gcccgatctg
     2641 cacgtgaagc tgcaatcgca ttcatccacc tcaacgaccc cacgactgag gcagcagcgc
     2701 accacgcgtc ccaggcctaa gacaattcac gtggatgatg ccagcgtgga catcagcgag
     2761 gcttcaagca tctctagtcg gggcaaaaaa ggctcaagct cgaatctaac ctga
//
