LOCUS       sequence-9241-ex        5053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5053)
  TITLE     Forth generation Seydoux lab vectors
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCM3.17
FEATURES             Location/Qualifiers
     source          1..5053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3229..3328)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3440..3459)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3451..4260
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3518..3537)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4403..4991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4892..4911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatggagtt gggctcaccg aacgatgcaa actacactgt attctgctgt
      481 tctcatgtcg ttttggcttt tgccattttt atggtaattt ttggaagtgt tctgggataa
      541 tttttggaat tttggaatta aaaaattttt tgaaaatttt gggaaaattt tttttttttt
      601 caaaaatttc aaaacgtttt tttcactttt ttttttgaat ttcaaatatt ttccagatcc
      661 ttgactttac cctaactcta ttctcatttg gcttagagct tttcctcatt ttcgtaagtt
      721 tttctcctga aagttcccct caaaatcccc aattttcctg cagttccacg acacttttga
      781 ccaattttgg gcgatttttc acggaatttc actggttact tcaatttccg cagttttggc
      841 gatttgtgtg agtatatttg gcggaaaatt tgagaaaatc tgaaattttt gacttaaatt
      901 ttaggaaatt ttttttctta atctgaaatt ttgaagctgt tttcccaaaa aaatcaataa
      961 aaaagcctac gatttccata attaccaaaa aaaaaatcca aaatttcctc atttacactc
     1021 aaaaaaaccg taaaaccccg agattgaagc tcagactaca aactacaatg ttggttgagg
     1081 ctgagcgcag aattgtagtt tgtagtctga gcctcaccca acataattat atttcaggat
     1141 aatccagtgg caattattgt ttatgggtgt cgtgttatgt tgattttggg attcgttaca
     1201 actcggttca tgtgagtttg actacaaact acaaactaca aactacaaac tacaaactac
     1261 aaactacaaa atgcaaacta caaaatgcaa actacaaagt acaaactaca aactacaact
     1321 gggaggaaca agcttgctga gactacaaac cacaatttgg ggttgcaact caaaaagttg
     1381 tagtctgtag ttagtttgta gtttgtagtt tgtagtttgt agcttgtagt ttgtagtgtg
     1441 cactctgttt tttgtagttt gtaacttgca agtcatatta gaaacatatt agcaattttc
     1501 caaagtttca aaattctcac ctaatttcgc aataccgata tcagtacaat aagtcttatg
     1561 gaagttgtag tttgtagttt atagtttgta gtttgtagtt tgtagtttgt agcctgcaag
     1621 tgatgttcga gacgtattgg caattttcca aaatttcaaa tttcttacct aatttctcta
     1681 aaccaatact agtgcaatac gtgttaggga aatagtagtt tgtagtttgt agtctgtagt
     1741 atgtagtttg tagtttgaat tttgtagctt gaaagtcata ttcgagacat attgaggctt
     1801 ttccaaaatt tcaaatttct cacctaattt cacaataccg atatcagtga aatacgtgtt
     1861 aaggaaattg tagtttgtag tttctagtgt gtagtttgta gtttgtagcc cttccaaccc
     1921 atttccaaac ttcagaatct caatgcgtga tttcacatca aaagaagagc caactctgag
     1981 tgaattgttg aatttattcc aaatcgtgat tgtgataatg tacacaattg gcattttcct
     2041 agtgattcgt ctatttacct attcagctgg aagatatcgt ggatattatg acgtggatga
     2101 tgatttgaaa gacgaaattc gaaagtatgt ttttagggat tttgaaaaat tgaaaaaaat
     2161 acgttttttt cagaaaattc ctggagtatg aggcggattt ggatgagaaa tcagagaaga
     2221 aggattaatt tttttaaact tttgaatgat ttttaataaa atggctgagt agtttgggtt
     2281 ttcaaccgga attcaaattt tccttcaatt tttttctttc tgctcattga acaggtctca
     2341 taagctcgag aaacttctgg aatggataat tttttgactc tgtcgaaaaa gtgtcaatat
     2401 taatgcaaat tatgaaaatt tattctcaga aacaattaca atcatgtatt ataaacgttt
     2461 ctgccattca ccaagcagtc ggtacaactt ttttttaccc aacacgattc cggacttctt
     2521 gctccagcgc agtttgcaat ccaaccatca cttctcacat ctttatcaca agtttccgca
     2581 cattttggtc tttcagcttt tggacgtagt tcagaacacg caatacggca ataatcgtac
     2641 cgataggtat aggcattaca ggccgagagg cacaacagca ggaatagtac gattttggtt
     2701 atcattatga tgaagtagaa tatggtgata tttaaaattt taaaaacgtg gaatttgagt
     2761 tattcgtttt taagacaatt gttatgctac ttaggccccg tcaactgtca atgtaaacaa
     2821 ttatgtctag accggaagct tatcagctgc acggcctgta attaggctcc tctgaccaaa
     2881 actacagggg taggcggctt aaaaccgatt taaccgaatt cgccgatcgg ctaacttggc
     2941 taactcggtt aatattagct aatattaaat aatttttgta gcaataccaa aaaatttttg
     3001 tccgaatgtg ccaaattaat ttattgaact tcattgcaac aaattaccct aaaaattgtg
     3061 attttcttat tagacaaccc gagttataag gtatttgaaa aatttgagaa aaaataaagt
     3121 aaccttactt caaattgtca tccatggtag aaaatgtttt caaatacaac aaattccata
     3181 taagtttcta aaaaaattat gaaaaactga aaaatcgtcc atgatgttac ccagctttct
     3241 tgtacaaagt gggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     3301 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
     3361 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     3421 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     3481 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3541 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3601 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3661 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3721 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3781 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3841 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3901 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3961 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     4021 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     4081 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     4141 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     4201 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     4261 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     4321 gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4381 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4441 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4501 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4561 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4621 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4681 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4741 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4801 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4861 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4921 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4981 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5041 ttgctcacat gtt
//
