LOCUS       sequence-92410-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of AP1S3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Setta-Kaffetzi N, Simpson MA, Navarini AA, Patel VM, Lu HC, Allen 
            MH, Duckworth M, Bachelez H, Burden AD, Choon SE, Griffiths CE, 
            Kirby B, Kolios A, Seyger MM, Prins C, Smahi A, Trembath RC, 
            Fraternali F, Smith CH, Barker JN, Capon F
  TITLE     AP1S3 mutations are associated with pustular psoriasis and impaired 
            Toll-like receptor 3 trafficking.
  JOURNAL   Am J Hum Genet. 2014 May 1;94(5):790-7. doi: 
            10.1016/j.ajhg.2014.04.005.
  PUBMED    24791904
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ajhg.2014.04"; journalName: "Am J Hum Genet"; date: 
            "2014-05-1- 1"; volume: "94"; issue: "5"; pages: "790-7"
COMMENT     Sequence Label: pcDNA3.1-myc-AP1S3
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(52..81)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(641..660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(642..660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggagaccgtt taaactcaat gatgatgatg atgatggtcg acggcgctat tcagatcctc
       61 ttctgagatg agtttttgtt cgggcccaag cttggtacca aatgtaggct tgttcatgta
      121 ttcttccatt gtctcctgta acatatcaga gtcttcaatg gctttgacag caattttctt
      181 ggatgtttcc tgaatttccc cacctattat aaactcgtcc aggatgaaat aagccttttc
      241 aaaattaaag ataatatcca gctcacagac atttccaaaa tatttgtcca gcagctccac
      301 gtaacgatgc acaatctcta gcgtcaagag ctcattgtcc tgattttcta ttgcacagca
      361 aaaatataaa ctagcatacc ttttataaac aagttttagc tccttccagt caacaaaact
      421 gcttgtcctg tgaccacggg agagaataat ctgaacaatt tcccgggtga tcttcttcct
      481 ctctttatca gggagagtga tgtaccattt ctgtagccgt aatttccctt gtcgactgaa
      541 gagcaatatg aaatgtatca tcggaattct gcagatatcc agcacagtgg cggccgctcg
      601 agtctagagg gcccgtttaa acgctagcca gcttgggtct ccctatagtg agtcgtatta
      661 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag
      721 acctcccac
//
