LOCUS       sequence-92421-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-sfGFP-DocI
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..31
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             46..68
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             118..141
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tataggggaa ttgtgagcgg ataacaattc ccctgtagaa ataattttgt ttaactttaa
       61 gaaggagata tacatatggg taccgactct ctggaattca tcgcttctaa actggctctg
      121 gaagttctgt tccagggtcc gctgcagcac caccaccacc accacccatg gactagtgct
      181 agcagcaaag gtgaagaact gtttaccggt gttgttccga ttctggttga actggatggt
      241 gatgttaatg gccacaaatt ttcagttcgt ggtgaaggcg aaggtgatgc aaccattggt
      301 aaactgaccc tgaaatttat ctgtaccacc ggcaaactgc cggttccgtg gccgaccctg
      361 gttaccaccc tgacctatgg tgttcagtgt tttagccgtt atccggatca tatgaaacgc
      421 cacgattttt tcaaaagcgc aatgccggaa ggttatgttc aagaacgtac catctccttt
      481 aaagacgacg gtaaatacaa aacccgtgcc gttgttaaat ttgaaggtga taccctggtg
      541 aatcgcattg aactgaaagg caccgatttt aaagaggatg gtaatatcct gggccacaaa
      601 ctggaatata atttcaatag ccacaacgtg tatatcaccg cagacaaaca gaaaaatggc
      661 atcaaagcca attttaccgt gcgccataat gttgaagatg gtagcgtgca gctggcagat
      721 cat
//
