LOCUS       sequence-92426-e        5722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses lncRNA MEG3 isoform a in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, 
            Zhao J, Weng C, Klibanski A
  TITLE     Activation of p53 by MEG3 non-coding RNA.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24731-42. Epub 2007 Jun 13.
  PUBMED    17569660
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24731-42. 
            Epub 2007 Jun 13"
COMMENT     Sequence Label: pCI-MEG3a
FEATURES             Location/Qualifiers
     source          1..5722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2836..2957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2847..2866)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2901..2920
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3138..3593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3225..3244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3435..3456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3660..3679)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3779..3801
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3839..3857)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3925..4029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4030..4098,4099..4890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4248..4267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5061..5649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5550..5569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcgc agagagggag
     1081 cgcgccttgg ctcgctggcc ttggcggcgg ctcctcagga gagctggggc gcccacgaga
     1141 ggatccctca cccgggtctc tcctcaggga tgacatcatc cgtccacctc cttgtcttca
     1201 aggaccacct cctctccatg ctgagctgct gccaaggggc ctgctgccca tctacacctc
     1261 acgagggcac taggagcacg gtttcctgga tcccaccaac atacaaagca gccactcact
     1321 gacccccagg accaggatgg caaaggatga agaggaccgg aactgaccag ccagctgtcc
     1381 ctcttaccta aagacttaaa ccaatgccct agtgaggggg cattgggcat taagccctga
     1441 cctttgctat gctcatactt tgactctatg agtactttcc tataagtctt tgcttgtgtt
     1501 cacctgctag caaactggag tgtttccctc cccaaggggg tgtcagtctt tgtcgactga
     1561 ctctgtcatc acccttatga tgtcctgaat ggaaggatcc ctttgggaaa ttctcaggag
     1621 ggggacctgg gccaagggct tggccagcat cctgctggca actccaaggc cctgggtggg
     1681 cttctggaat gagcatgcta ctgaatcacc aaaggcacgc ccgacctctc tgaagatctt
     1741 cctatccttt tctgggggaa tggggtcgat gagagcaacc tcctagggtt gttgtgagaa
     1801 ttaaatgaga taaaagaggc ctcaggcagg atctggcata gaggaggtga tcagcaaatg
     1861 tttgttgaaa aggtttgaca ggtcagtccc ttcccacccc tcttgcttgt cttacttgtc
     1921 ttatttattc tccaacagca ctccaggcag cccttgtcca cgggctctcc ttgcatcagc
     1981 caagcttctt gaaaggcctg tctacacttg ctgtcttcct tcctcacctc caatttcctc
     2041 ttcaacccac tgcttcctga ctcgctctac tccgtggaag cacgctcaca aaggttccca
     2101 gtgcccccga caagcccctg ctggtgtctc catctcctgc caagcatcct ccagtgcctc
     2161 ctcctgtggg cctggcctca gggctatgga cagactcctg tcccatccca gagacccctc
     2221 gtgatcgttc cctggcacgt gggccgtggc ccggctgggt cggctgaaga actgcggatg
     2281 gaagctgcgg aagaggccct gatggggccc accatcccgg acccaagtct tcttcctggc
     2341 gggcctctcg tctccttcct ggtttgggcg gaagccatca cctggatgcc tacgtgggaa
     2401 gggacctcga atgtgggacc ccagcccctc tccagctcga aatccctcca cagccacggg
     2461 gacaccctgc acctattccc acgggacagg ctggacccag agactctgga cccggggcct
     2521 ccccttgagt agagacccgc cctctgactg atggacgccg ctgacctggg gtcagacccg
     2581 tgggctggac ccctgcccac cccgcaggaa ccctgaggcc taggggagct gttgagcctt
     2641 cagtgtctgc atgtgggaag tgggctcctt cacctacctc acagggctgt tgtgaggggc
     2701 gctgtgatgc ggttccaaag cacagggctt ggcgcacccc actgtgctct caataaatgt
     2761 gtttcctgtc ttaacaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagcggccgc
     2821 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2881 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     2941 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3001 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     3061 atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3121 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3481 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3541 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     3601 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     3661 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3721 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3781 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     3841 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3901 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3961 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4561 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5701 gctcacatgg ctcgacagat ct
//
