LOCUS       sequence-92427-e        5565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses lncRNA MEG3 isoform b in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, 
            Zhao J, Weng C, Klibanski A
  TITLE     Activation of p53 by MEG3 non-coding RNA.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24731-42. Epub 2007 Jun 13.
  PUBMED    17569660
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24731-42. 
            Epub 2007 Jun 13"
COMMENT     Sequence Label: pCI-MEG3b
FEATURES             Location/Qualifiers
     source          1..5565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2679..2800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2690..2709)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2744..2763
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2981..3436
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3068..3087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3503..3522)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3622..3644
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3682..3700)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3768..3872
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3873..3941,3942..4733)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4091..4110)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4904..5492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5393..5412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattccg cgccttggct
     1081 cgctggcctt ggcggcggct cctcaggaga gctggggcgc ccacgagagg atccctcacc
     1141 cgggtctctc ctcagggatg acatcatccg tccacctcct tgtcttcaag gaccacctcc
     1201 tctccatgct gagctgctgc caaggggcct gctgcccatc tacacctcac gagggcacta
     1261 ggagcacggt ttcctggatc ccaccaacat acaaagcagc cactcactga cccccaggac
     1321 caggatggca aaggatgaag aggaccggaa ctgaccagcc agctgtccct cttacctaaa
     1381 gacttaaacc aatgccctag tgagggggca ttgggcatta agccctgacc tttgctatgc
     1441 tcatactttg actctatgag tacttcccta taagtctttg cttgtgttca cctgctagca
     1501 aactggagtg tttccctccc caagggggtg tcagtctttg tcgactgact ctgtcatcac
     1561 ccttatgatg tcctgaatgg aaggatccct ttgggaaatt ctcaggaggg ggacctgggc
     1621 caagggcttg gccagcatcc tgctggcaac tccaaggccc tgggtgggct tctggaatga
     1681 gcatgctact gaatcaccaa aggcacgccc gacctctctg aagatcttcc tatccttttc
     1741 tgggggaatg gggtcgatga gagcaacctc ctagggttgt tgtgagaatt aaatgagata
     1801 aaagaggcct caggcaggat ctggcataga ggaggtgatc agcaaatgtt tgttgaaaag
     1861 gtttgacagg tcagtccctt cccacccctc ttgcttgtct tacttgtctt atttattctc
     1921 caacagcact ccaggcagcc cttgtccacg ggctctcctt gcatcagggc taatctcggg
     1981 ccttgtcgaa ggaagaggct gcagacgtta atgaggttag ctgctggatt ccagtattcg
     2041 tcgcataagg atccttcttt gtctgcgaag gaaaaacaca ctgattatca taatgaggca
     2101 cgtgggccgt ggcccggctg ggtcggctga agaactgcgg atggaagctg cggaagaggc
     2161 cctgatgggg cccaccatcc cggacccaag tcttcttcct ggcgggcctc tcgtctcctt
     2221 cctggtttgg gcggaagcca tcacctggat gcctacgtgg gaagggacct cgaatgtggg
     2281 accccagccc ctctccagct cgaaatccct ccacagccac ggggacaccc tgcacctatt
     2341 cccacgggac aggctggacc cagagactct ggacccgggg cctccccttg agtagagacc
     2401 cgccctctga ctgatggacg ccgctgacct ggggtcagac ccgtgggctg gacccctgcc
     2461 caccccgcag gaaccctgag gcctagggga gctgttgagc cttcagtgtc tgcatgtggg
     2521 aagtgggctc cttcacctac ctcacagggc tgttgtgagg ggcgctgtga tgcggttcca
     2581 aagcacaggg cttggcgcac cccactgtgc tctcaataaa tgtgtttcct gtcttaacaa
     2641 aaaaaaaaaa aaaaagcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     2701 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2761 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2821 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
     2881 caaatgtggt aaaatcgata aggatccggg ctggcgtaat agcgaagagg cccgcaccga
     2941 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     3001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3181 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3241 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3301 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3361 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3421 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3481 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     3541 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3601 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3661 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3721 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3781 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3841 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3901 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3961 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4021 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4081 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4141 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4201 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4261 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4321 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4381 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4441 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4501 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4561 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4621 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4681 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4741 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4801 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5521 tcctggcctt ttgctggcct tttgctcaca tggctcgaca gatct
//
