LOCUS       sequence-9243-ex        4668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC94
FEATURES             Location/Qualifiers
     source          1..4668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             500..1771
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        2413..2517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2518..2586,2587..3378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2736..2755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3549..4137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4038..4057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4291..4308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4425..4446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4461..4466,4467..4484,4485..4491)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4499..4515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4504..4526
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4523..4539
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4523..4539
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcaagag catttgaatc agaatatgga
       61 gaacggagca tgagcatttt cgaagttttt tagatgcact agaacaaagc gtgttggctt
      121 cctctgagcc cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag
      181 gtcctagatg cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa
      241 tgtatctggg tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct
      301 ccttttgcaa caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt
      361 tgaaaataaa taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaaactata
      421 atcatctcac tggatcctat cgattcgcgg ccgctgtaca cccgggtgcc ctatagtgag
      481 tcgtattggt accaaaaaca tgccacaatt tgatatatta tgtaaaacac cacctaaggt
      541 gcttgttcgt cagtttgtgg aaaggtttga aagaccttca ggtgagaaaa tagcattatg
      601 tgctgctgaa ctaacctatt tatgttggat gattacacat aacggaacag caatcaagag
      661 agccacattc atgagctata atactatcat aagcaattcg ctgagtttcg atattgtcaa
      721 taaatcactc cagtttaaat acaagacgca aaaagcaaca attctggaag cctcattaaa
      781 gaaattgatt cctgcttggg aatttacaat tattccttac tatggacaaa aacatcaatc
      841 tgatatcact gatattgtaa gtagtttgca attacagttc gaatcatcgg aagaagcaga
      901 taagggaaat agccacagta aaaaaatgct taaagcactt ctaagtgagg gtgaaagcat
      961 ctgggagatc actgagaaaa tactaaattc gtttgagtat acttcgagat ttacaaaaac
     1021 aaaaacttta taccaattcc tcttcctagc tactttcatc aattgtggaa gattcagcga
     1081 tattaagaac gttgatccga aatcatttaa attagtccaa aataagtatc tgggagtaat
     1141 aatccagtgt ttagtgacag agacaaagac aagcgttagt aggcacatat acttctttag
     1201 cgcaaggggt aggatcgatc cacttgtata tttggatgaa tttttgagga attctgaacc
     1261 agtcctaaaa cgagtaaata ggaccggcaa ttcttcaagc aataaacagg aataccaatt
     1321 attaaaagat aacttagtca gatcgtacaa taaagctttg aagaaaaatg cgccttattc
     1381 aatctttgct ataaaaaatg gcccaaaatc tcacattgga agacatttga tgacctcatt
     1441 tctttcaatg aagggcctaa cggagttgac taatgttgtg ggaaattgga gcgataagcg
     1501 tgcttctgcc gtggccagga caacgtatac tcatcagata acagcaatac ctgatcacta
     1561 cttcgcacta gtttctcggt actatgcata tgatccaata tcaaaggaaa tgatagcatt
     1621 gaaggatgag actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc
     1681 tgaaggaagc atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta
     1741 cctttcatcc tacataaata gacgcatata agtacgcatt taagcataaa cacgcactat
     1801 gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac
     1861 agtgagctgt atgtgcgggg atccactagt tctagccggc catacaagta atccggatga
     1921 tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta
     1981 ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa
     2041 atctaaacat gagcggtttt ttttctcttt ccgtctccca attcgtattc cgctcctctc
     2101 atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg
     2161 ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa
     2221 tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat
     2281 atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat
     2341 tgtcctcgct tgggcccaaa aggccttttt tttttggcgc cgacgtcagg tggcactttt
     2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2761 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3061 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3661 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3901 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4321 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4381 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4441 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4501 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa
     4561 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
     4621 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaat
//
