LOCUS       sequence-92433-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probing molecule to perform single molecule force spectroscopy 
            experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-CBM-CohI
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..36
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             123..146
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcactatag gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgggtaccg actctctgga attcatcgct tctaaactgg
      121 ctctggaagt tctgttccag ggtccgctgc agcaccacca ccaccaccac ccatggacta
      181 gtgctagcat gtgcaataca gtatcaggca atttgaaggt tgaattctac aacagcaatc
      241 cttcagatac tactaactca atcaatcctc agttcaaggt tactaatacc ggaagcagtg
      301 caattgattt gtccaaactc acattgagat attattatac agtagacgga cagaaagatc
      361 agaccttctg gtgtgaccat gctgcaataa tcggcagtaa cggcagctac aacggaatta
      421 cttcaaatgt aaaaggaaca tttgtaaaaa tgagttcctc aacaaataac gcagacacct
      481 accttgaaat aagctttaca ggcggaactc ttgaaccggg tgcacatgtt cagatacaag
      541 gtagatttgc aaagaatgac tggagtaact atacacagtc aaatgactac tcattcaagt
      601 ctgcttcaca gtttgttgaa tgggatcagg taacagcata cttgaacggt gttcttgtat
      661 ggggtaaaga acccggtggc agtgtagtac catcaacaca gcctgtaaca acaccacctg
      721 caacaacaaa accacctgca acaacaatac cgccgtcaga tgatccgaat gcaggatccg
      781 acggtgtg
//
