LOCUS       sequence-92436-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Detection construct to label dockerin type I containing fusion 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-StrepII-TagRFP-CohI
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             138..161
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctgtagaaa taattttgtt taactttaag
      121 aaggagatat acatatgtgg tctcacccgc agttcgaaaa agtttctaaa ggtgaagaac
      181 tgatcaaaga aaacatgcac atgaaactgt acatggaagg tactgttaac aaccaccact
      241 tcaaatgcac ctctgaaggt gaaggtaaac cgtacgaagg tactcagacc atgcgtatca
      301 aagttgttga aggtggtccg ctgccgttcg ctttcgacat cctggctacc tctttcatgt
      361 acggttctcg taccttcatc aaccacaccc agggtatccc ggacttcttc aaacagtctt
      421 tcccggaagg tttcacctgg gaacgtgtta ccacctacga agacggtggt gttctgaccg
      481 ctacccagga cacctctctg caagacggtt gcctgatcta caacgttaaa atccgtggtg
      541 ttaacttccc gtctaacggt ccggttatgc agaaaaaaac cctgggttgg gaagctaaca
      601 ccgaaatgct gtacccggct gacggtggtc tggaaggtcg ttctgacatg gctctgaaac
      661 tggttggtgg tggtcacctg atctgcaact tcaaaaccac ctaccgttct aaaaaaccgg
      721 ctaaaaacct gaaaatgccg ggtgtttact acgttgacca ccgtctggaa cgtatcaaa
//
