LOCUS       sequence-92437-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-Xyl-DocI
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             182..205
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             212..229
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgggtaccga ctctctggaa ttcatcgctt ctaaactggc
      181 tctggaagtt ctgttccagg gtccgctgca gcaccaccac caccaccacc catggactag
      241 tgctagcaag aatgcagatt cctatgcgaa aaaacctcac atcagcgcat tgaatgcccc
      301 acaattggat caacgctaca aaaacgagtt cacgattggt gcggcagtag aaccttatca
      361 actacaaaat gaaaaagacg tacaaatgct aaagcgccac ttcaacagca ttgttgccga
      421 gaacgtaatg aaaccgatca gcattcaacc tgaggaagga aaattcaatt ttgaacaagc
      481 ggatcgaatt gtgaagttcg ctaaggcaaa tggcatggat attcgcttcc atacactcgt
      541 ttggcacagc caagtacctc aatggttctt tcttgacaag gaaggtaagc caatggttaa
      601 tgaatgcgat ccagtgaaac gtgaacaaaa taaacaactg ctgttaaaac gacttgaaac
      661 tcatattaaa acgatcgtcg agcggtacaa agatgacatt aagtactggg acgttgtaaa
      721 tgaggttgtg ggggacgacg gaaaactgcg caactctcca tggtatcaaa tcgccggcat
      781 cgattatatt aaagtggcat tccaagcagc tagaaaatat ggcggagaca acattaagct
      841 ttacatgaat gatta
//
