LOCUS       sequence-92438-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-10FNIII(x4)-DocI
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    60..84
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             99..121
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             171..194
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg
       61 gaattgtgag cggataacaa ttcccctgta gaaataattt tgtttaactt taagaaggag
      121 atatacatat gggtaccgac tctctggaat tcatcgcttc taaactggct ctggaagttc
      181 tgttccaggg tccgctgcag caccaccacc accaccaccc atggactagt gctagcgtta
      241 gtgatgttcc gcgtgatctg gaagttgttg cagcaacccc gaccagcctg ctgattagct
      301 gggatgcacc ggcagttacc gttcgttatt atcgtattac ctatggtgaa accggtggta
      361 atagtccggt tcaagaattt accgttccgg gtagcaaaag caccgcaacc attagcggtc
      421 tgaaaccggg tgttgattac accattaccg tttatgccgt taccggtcgt ggtgattcac
      481 cggcaagcaa caaaccgatt agcattaact atcgtaccgg tagcggtagt ggtagcgttt
      541 cagatgtgcc tcgcgacctg gaagtggtgg ctgccacacc gacctcactg ctgatctcat
      601 gggatgcccc tgccgtgacc gtgcgctatt atcgcatcac atatggcgag acaggtggca
      661 attcacctgt gcaagaattc acagttcctg gttcaaaaag taccgccaca atttctggcc
      721 tgaaacctgg cgtggattac acaa
//
