LOCUS       sequence-92445-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Characteristic fingerprint molecule to perform single molecule force
            spectroscopy experiments; covalently immobilized via ybbr tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-ybbR-HIS-sfGFP-DocI
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(278..325)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 tagcagccgg atcttactcg agttagttct tgtacggcaa tgtatctatt tctttgagaa
       61 tatatctctt caaaattcct aagtcagttg aattaactct gccgtcttca ttcaaatcgg
      121 cattgtcagt gttgatgctt atacctgatc tcaaaacata tctcttcaat gctacagcgt
      181 cagttgagtt aacttttccg tcatcattga cgtcgccgta taatttagta gaaggagtac
      241 caggtaccac tttcttatac agctcatcca taccatgggt aatacctgct gcattaacat
      301 actcatgcag caccatatga tcacgttttt cattcggatc tttgctcaga acggtctggg
      361 tgctcagata atgattatcc ggcagcagaa ccggaccatc accaatcggg gtattctgct
      421 gataatgatc tgccagctgc acgctaccat cttcaacatt atggcgcacg gtaaaattgg
      481 ctttgatgcc atttttctgt ttgtctgcgg tgatatacac gttgtggcta ttgaaattat
      541 attccagttt gtggcccagg atattaccat cctctttaaa atcggtgcct ttcagttcaa
      601 tgcgattcac cagggtatca ccttcaaatt taacaacggc acgggttttg tatttaccgt
      661 cgtctttaaa ggagatggta cgttcttgaa cataaccttc cggcattgcg cttttgaaaa
      721 aatcgtggcg tttcatatga tccggataac ggctaaaaca ctgaacacca taggtcaggg
      781 tggtaaccag ggtcggccac ggaaccggca gtttgccggt ggtacaga
//
