LOCUS       sequence-92470-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of AP1S3 R33W mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Setta-Kaffetzi N, Simpson MA, Navarini AA, Patel VM, Lu HC, Allen 
            MH, Duckworth M, Bachelez H, Burden AD, Choon SE, Griffiths CE, 
            Kirby B, Kolios A, Seyger MM, Prins C, Smahi A, Trembath RC, 
            Fraternali F, Smith CH, Barker JN, Capon F
  TITLE     AP1S3 mutations are associated with pustular psoriasis and impaired 
            Toll-like receptor 3 trafficking.
  JOURNAL   Am J Hum Genet. 2014 May 1;94(5):790-7. doi: 
            10.1016/j.ajhg.2014.04.005.
  PUBMED    24791904
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ajhg.2014.04"; journalName: "Am J Hum Genet"; date: 
            "2014-05-1- 1"; volume: "94"; issue: "5"; pages: "790-7"
COMMENT     Sequence Label: pcDNA3.1-myc-AP1S3 R33W
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(59..88)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(648..667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(649..667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(712..763)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(741..761)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttaagctgga gaccgtttaa actcaatgat gatgatgatg atggtcgacg gcgctattca
       61 gatcctcttc tgagatgagt ttttgttcgg gcccaagctt ggtaccaaat gtaggcttgt
      121 tcatgtattc ttccattgtc tcctgtaaca tatcagagtc ttcaatggct ttgacagcaa
      181 ttttcttgga tgtttcctga atttccccac ctattataaa ctcgtccagg atgaaataag
      241 ccttttcaaa attaaagata atatccagct cacagacatt tccaaaatat ttgtccagca
      301 gctccacgta acgatgcaca atctctagcg tcaagagctc attgtcctga ttttctattg
      361 cacagcaaaa atataaacta gcataccttt tataaacaag ttttagctcc ttccagtcaa
      421 caaaactgct tgtcctgtga ccacgggaga gaataatctg aacaatttcc caggtgatct
      481 tcttcctctc tttatcaggg agagtgatgt accatttctg tagccgtaat ttcccttgtc
      541 gactgaagag caatatgaaa tgtatcatcg gaattctgca gatatccagc acagtggcgg
      601 ccgctcgagt ctagagggcc cgtttaaacg ctagccagct tgggtctccc tatagtgagt
      661 cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct
      721 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      781 acgacatttt ggaaagtccc gttgattt
//
