LOCUS       sequence-92471-e         452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  is a lentiviral vector coding the shRNA si2 (sip1090) and the gene 
            of resistance to puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 452)
  TITLE     biG-biS plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psp-161-si2(sip1090)
FEATURES             Location/Qualifiers
     source          1..452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..175
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        183..397
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     318..337
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
       61 gacggtatcg gatcccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      121 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctggaat
      181 tcgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg
      241 aacacccagc gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc
      301 cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt
      361 tggatttggg aatcttataa gttctgtatg agaccacaga tccccggaca cctctcaagc
      421 atcattcaag agatgatgct tgagaggtgt cc
//
