LOCUS       sequence-92475-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN K35R mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN Kappa-35R
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             602..631
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             650..673
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(693..709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac tcactatagg
       61 gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg cgatcgccat
      121 ggtcctgagt ggggcgctgt gcttccgaat gaaggactcg gcattgaagg tgctttatct
      181 gcataataac cagcttctag ctggagggct gcatgcaggg agggtcatta aaggtgaaga
      241 gatcagcgtg gtccccaatc ggtggctgga tgccagcctg tcccccgtca tcctgggtgt
      301 ccagggtgga agccagtgcc tgtcatgtgg ggtggggcag gagccgactc taacactaga
      361 gccagtgaac atcatggagc tctatcttgg tgccaaggaa tccaagagct tcaccttcta
      421 ccggcgggac atggggctca cctccagctt cgagtcggct gcctacccgg gctggttcct
      481 gtgcacggtg cctgaagccg atcagcctgt cagactcacc cagcttcccg agaatggtgg
      541 ctggaatgcc cccatcacag acttctactt ccagcagtgt gacacgcgta cgcggccgct
      601 cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg attacaagga
      661 tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga acagatcccg
      721 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
      781 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
      841 tctataatat tatggggtgg aaggggg
//
