LOCUS       sequence-92477-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for human IL36RN K35R mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Hassi NK, Weston T, Rinaldi G, Ng JC, Smahi A, Twelves S, 
            Davan-Wetton C, Fakhreddine D, Fraternali F, Capon F
  TITLE     In Silico and In Vitro Analysis of IL36RN Alterations Reveals 
            Critical Residues for the Function of the Interleukin-36 Receptor 
            Complex.
  JOURNAL   J Invest Dermatol. 2023 Dec;143(12):2468-2475.e6. doi: 
            10.1016/j.jid.2023.06.191. Epub 2023 Jul 4.
  PUBMED    37414245
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.06.191"; journalName: "J Invest Dermatol"; date:
            "2023-12"; volume: "143"; issue: "12"; pages: "2468-2475.e6"
COMMENT     Sequence Label: pCMV6-Entry IL36RN Kappa-35R
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..75
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     59..75
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(95..118)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(137..166)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(707..726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(708..726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(752..955)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(781..801)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttccagggcc aggagaggca ctggggaggg gtcacaggga tgccacccgg gatctgttca
       61 ggaaacagct atgaccgcgg ccggccgttt aaaccttatc gtcgtcatcc ttgtaatcca
      121 ggatatcatt tgctgccaga tcctcttctg agatgagttt ctgctcgagc ggccgcgtac
      181 gcgtgtcaca ctgctggaag tagaagtctg tgatgggggc attccagcca ccattctcgg
      241 gaagctgggt gagtctgaca ggctgatcgg cttcaggcac cgtgcacagg aaccagcccg
      301 ggtaggcagc cgactcgaag ctggaggtga gccccatgtc ccgccggtag aaggtgaagc
      361 tcttggattc cttggcacca agatagagct ccatgatgtt cactggctct agtgttagag
      421 tcggctcctg ccccacccca catgacaggc actggcttcc accctggaca cccaggatga
      481 cgggggacag gctggcatcc agccaccgat tggggaccac gctgatctct tcacctttaa
      541 tgaccctccc tgcatgcagc cctccagcta gaagctggtt attatgcaga taaagcacct
      601 tcaatgccga gtccttcatt cggaagcaca gcgccccact caggaccatg gcgatcgcgg
      661 cggcagatct cctcggtacc ggatccagtc gacgaattcc cggccgccct atagtgagtc
      721 gtattacaaa attctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
      781 cacgcctacc gcccatttgc gtcaacgggg cggggttatt acgacatttt ggaaagtccc
      841 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
      901 cccgtgagtc aaaccgctat ccacgcccat tggtgtactg ccaaaaccgc atcaccatgg
      961 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccgt aaggtcatgt
     1021 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
//
