LOCUS       sequence-92494-e       10757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  silence doxycyclin inducible pUltra-dox (also expresses luciferase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10757)
  TITLE     pUltra plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Smurf-luc-RTTA3
FEATURES             Location/Qualifiers
     source          1..10757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             236..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1835..1952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1999..2032
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        2064..3275
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3112..3131
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3307..3993
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     CDS             4012..4068
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4075..5724
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4141..4159)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             5740..5793
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5800..6417,6418..6423,6424..6459,6460..6462,6463..6498,
                     6499..6501,6502..6537,6538..6546)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    6553..6586
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    6598..7186
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6651..6671)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7069..7080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7277..7510
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7582..7703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7619..7638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7673..7692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7743..7878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7898..7917)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7899..7917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7927..7944)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7927..7943)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7936..7958)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8088..8543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8175..8194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8385..8406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8572..8676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8677..8745,8746..9537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8895..8914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9708..10296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10197..10216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10450..10467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10584..10605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10620..10625,10626..10643,10644..10650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10658..10674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10663..10685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10682..10698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10682..10698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10717..10737
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10719..10737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     1801 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     1861 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     1921 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     1981 cagtttggtt aattaacgat aacttcgtat aatgtgtact atacgaagtt atctcgagat
     2041 taacccgtgt cggctccaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     2101 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     2161 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     2221 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2281 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2341 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2401 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2461 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2521 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2581 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2641 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2701 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2761 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2821 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2881 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2941 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     3001 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     3061 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     3121 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     3181 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3241 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtccgatcc
     3301 accggtatgg ccctgtccaa caagttcatc ggcgacgaca tgaagatgac ctaccacatg
     3361 gacggctgcg tgaacggcca ctacttcacc gtgaagggcg agggcagcgg caagccctac
     3421 gagggcaccc agacctccac cttcaaggtg accatggcca acggcggccc cctggccttc
     3481 tccttcgaca tcctgtccac cgtgttcatg tacggcaacc gctgcttcac cgcctacccc
     3541 accagcatgc ccgactactt caagcaggcc ttccccgacg gcatgtccta cgagagaacc
     3601 ttcacctacg aggacggcgg cgtggccacc gccagctggg agatcagcct gaagggcaac
     3661 tgcttcgagc acaagtccac cttccacggc gtgaacttcc ccgccgacgg ccccgtgatg
     3721 gccaagaaga ccaccggctg ggacccctcc ttcgagaaga tgaccgtgtg cgacggcatc
     3781 ttgaagggcg acgtgaccgc cttcctgatg ctgcagggcg gcggcaacta cagatgccag
     3841 ttccacacct cctacaagac caagaagccc gtgaccatgc cccccaacca cgtggtggag
     3901 caccgcatcg ccagaaccga cctggacaag ggcggcaaca gcgtgcagct gaccgagcac
     3961 gccgtggccc acatcacctc cgtggtgccc ttcgtgtaca agggatctgg agcaacaaac
     4021 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccgggccttc tagtatggaa
     4081 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
     4141 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     4201 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
     4261 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     4321 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     4381 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
     4441 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
     4501 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     4561 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
     4621 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
     4681 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
     4741 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     4801 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     4861 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
     4921 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
     4981 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
     5041 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
     5101 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     5161 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     5221 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
     5281 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     5341 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
     5401 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
     5461 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
     5521 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     5581 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     5641 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     5701 aagaagggcg gaaagatcgc cgtgagatcc ggctccggcg agggcagggg aagtctacta
     5761 acatgcgggg acgtggagga aaatcccggc ccagctagta tgtccagact ggacaagagc
     5821 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     5881 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     5941 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     6001 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     6061 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     6121 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     6181 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     6241 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     6301 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     6361 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     6421 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     6481 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     6541 gggtaagaat tcataacttc gtataatgtg tactatacga agttatgtcg actcgacaat
     6601 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6661 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6721 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6781 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6841 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6901 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6961 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7021 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7081 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcatcttcgc
     7141 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattcg
     7201 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7261 aaagaaaagg ggggactgga agggctacgt aactcccaac gaagacaaga tctgcttttt
     7321 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7381 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7441 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7501 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7561 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7681 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7861 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     7921 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7981 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8041 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
     8101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8161 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8221 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8281 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8401 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8461 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8521 caaaatatta acgtttacaa tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc
     8581 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8641 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8701 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8761 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8821 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8881 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8941 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9001 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9061 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9121 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9181 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9241 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9301 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9361 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9421 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9481 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9541 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9601 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10321 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10381 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10441 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10501 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10681 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    10741 caaaagctgg agctgca
//
