LOCUS       sequence-92522-e         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 carrying the Cyc1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pD221 Cyc1_term
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      92..339
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(108..126)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(333..432)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgcgttttt
       61 tataatgcca actttgtaca aaaaagcagg ctcatgtaat tagttatgtc acgcttacat
      121 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
      181 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
      241 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
      301 cttgagaagg ttttgggacg ctcgaaggct ttacccagct ttcttgtaca aagttggcat
      361 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      421 aatcattatt tg
//
