LOCUS       sequence-92527-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of bovine pancreatic ribonuclease A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT
  TITLE     Engineering ribonuclease A: production, purification and 
            characterization of wild-type enzyme and mutants at Gln11.
  JOURNAL   Protein Eng. 1995 Mar;8(3):261-73.
  PUBMED    7479688
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng."; date: "1995-03"; volume: "8(3)"; pages: "261-73"
COMMENT     Sequence Label: pET22b RNase A
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             57..79
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     87..152
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             153..197
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             557..574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
       61 tttaacttta agaaggagat atacatatga aatacctgct gccgaccgct gctgctggtc
      121 tgctgctcct cgctgcccag ccggcgatgg ccaaggaaac tgcagcagcc aagtttgagc
      181 ggcagcacat ggactccagc acttccgctg ccagcagctc caactactgt aaccagatga
      241 tgaagagccg gaacctgacc aaagatcgat gcaagccagt gaacaccttt gtgcacgagt
      301 ccctggctga tgtccaggcc gtgtgctccc agaaaaatgt tgcctgcaag aatgggcaga
      361 ccaattgcta ccagagctac tccaccatga gcatcaccga ctgccgtgag accggcagct
      421 ccaagtaccc caactgtgcc tacaagacca cccaggcgaa taaacacatc attgtggctt
      481 gtgagggaaa cccgtacgtg ccagtccact ttgatgcttc agtgtagtag tcgacaagct
      541 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta a
//
