LOCUS       sequence-92533-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human RdgBbeta splice variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Garner K, Li M, Ugwuanya N, Cockcroft S
  TITLE     The phosphatidylinositol transfer protein RdgBbeta binds 14-3-3 via 
            its unstructured C-terminus, whereas its lipid-binding domain 
            interacts with the integral membrane protein ATRAP (angiotensin II 
            type I receptor-associated protein).
  JOURNAL   Biochem J. 2011 Oct 1;439(1):97-111. doi: 10.1042/BJ20110649.
  PUBMED    21728994
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2011-10-1- 1"; volume: "439"; issue: "1"; pages:
            "97-111"
COMMENT     Sequence Label: pRSETC-RdgBbeta-splice variant 1
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..104
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(108..116,117..131)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc
       61 atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
      121 atgacgataa ggatcgatgg atccgacctc gagtgctgct gaaagagtac cggatctgca
      181 tgccgctcac cgtagacgag tacaaaattg gacagctgta catgatcagc aaacacagcc
      241 atgaacagag tgaccgggga gaaggggtgg aggtcgtcca gaatgagccc tttgaggacc
      301 ctcaccatgg caatgggcag ttcaccgaga agcgggtgta tctcaacagc aaactgccta
      361 gttgggctag agctgttgtc cccaaaatat tttatgtgac agagaaggct tggaactatt
      421 atccctacac aattacagaa tacacatgtt cctttctgcc gaaattctcc attcatatag
      481 aaaccaagta tgaggacaac aaaggaagca atgacaccat tttcgacagt gaagccaaag
      541 acgtggagag agaagtttgc tttattgata ttgcctgcga tgaaattcca gagcgctact
      601 acaaagaatc cgaggatcct aagcacttca agtcagagaa gacaggacgg ggacagttga
      661 gggaaggctg gagagatagt catcagccta tcatgtgctc ctacaagctg gtgactgtga
      721 agtctgaggt ctgggggctt cagaccagag tggaacaatt tgtacacaag gtggtccgag
      781 acattctgct gattggacat agacaggctt ttgcatgggt tgatgagtgg tatgacatga
      841 caatggatga agtccgagaa tttgaacgag ccactcagga agccaccaac aagaaaatcg
      901 gcattttccc acctgcaatt tctatctcca gcatccccct gctgccttct tccgtccgca
      961 gtgcgccttc tagtgctcca tccacccctc tctccacaga cgcacccgaa tttctgtccg
     1021 ttcccaaaga tcggccccgg aaaaagtctg ccccagaaac tctcacactt
//
