LOCUS       sequence-92534-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inhibition module. Constructed from the parent plasmids pLuxRI, 
            pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 
            lysozyme by inducing AHL and LuxR. Chloramphenicol resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Payne S, Li B, Cao Y, Schaeffer D, Ryser MD, You L
  TITLE     Temporal control of self-organized pattern formation without 
            morphogen gradients in bacteria.
  JOURNAL   Mol Syst Biol. 2013 Oct 8;9:697. doi: 10.1038/msb.2013.55.
  PUBMED    24104480
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-8"; volume: "9:697. doi"; pages: " 
            10.1038/msb.2013.55"
COMMENT     Sequence Label: pTuLys2CMR2
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(116..149)
                     /label=T7 Phi-3.8 promoter
                     /note="weak promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(147..602)
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
ORIGIN
        1 tgtggatccg gcccattggc tgcctcccac acttggatat gcctcctcgg agccttatag
       61 aattgtttat aagacttgcg cattatttga cctccaatgc gaacaaaggg aaaccgctgt
      121 ggtctccctt tagtgagttc aattaattat ccacggtcag aagtgaccag ttcgttcttc
      181 tcccaccaac gcttaaggtc gaacgaaggg caagccttcg gcgccacctc atgatgggcg
      241 cgaagcacag cgccttcgta cttagccagc agtgtgacaa gcagtgagcg aagggattgc
      301 atttgggctg gcgtaaagtt agcgtcgaac ttacctttat cgtcgatacc accaacaagg
      361 cagacgccga tagagttgtg gttgtaaccc ttagcgtgag agcctacagc catctcatct
      421 cgtcctgcct ccacagtacc gtctcgcttg atgataaagt ggtatcccac atcgagccaa
      481 ccctgctctt tgtgccactg gcgaatctca cggacaccaa cattctgact tggcttggta
      541 gccgagcagt gaacaaagat tgcgtcagta gattcacgtt gtttaaactg tacacgagcc
      601 attatttctt tcctcctttc cgctagctag tcaagctttt acttgtacag ctcgtccatg
      661 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
      721 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
      781 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
      841 a
//
