LOCUS       sequence-9254-ex       12386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 12386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC114
FEATURES             Location/Qualifiers
     source          1..12386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1504..1551)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1590..1610
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1653..1681)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2429..2449
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(3091..3102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5092..5139)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5256..5276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5366..5388)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9826..9848)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9948..9967
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10045..10063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10131..10235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10236..10304,10305..11096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10454..10473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11267..11855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11756..11775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12009..12026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12143..12164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12179..12184,12185..12202,12203..12209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12217..12233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12222..12244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12241..12257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12241..12257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgacgtt tctgatagtt actagctcga cataatcaaa
      481 atccaacgtt ttctcattca gtaaatccct ttttcgttcc aaataaagag gaagaagatg
      541 gtcatgacga cgtcgaatgt agtattgatg tgtcttggct gcttctgaaa tatttcgatg
      601 cggtatgaat ttaatctgta tatacctgtc ggtggtgtcc atccgcttgg tgtaggatac
      661 ttttcgtgct caggaacatt cggcaccact tctattggca tcaatctttc cacatacgac
      721 caatcgatag gagcttcttc gaccgttgca agacttctgg ctgtaattgc tagtagaaaa
      781 attatatgat aataaataac ttacactttt cagggagagc atgtttccat ggattttccc
      841 aaagtttctc agctttttcg actgaagttg ttgaaaaaga caacctggaa actgctcgag
      901 gggagaaaat gcggagagaa gaacccagca tcttaataaa attatataat tttgaataaa
      961 atcaaattaa aaataagatt aattgaaaca caacaataga atgcagaaaa ttactcataa
     1021 gaaataattt attttgaaag aagttgtgtc gatttacgat aaaaactaca cgcaaacgag
     1081 gctccgcgtt tacaccgttt ggcaatggag cgcgcttgct attagatttt aggccagctc
     1141 aacgaaagtt taatttattt ttcttctttt tttccatttt tttgagtaaa atatcgaatt
     1201 ttttgtatta attctgtaaa ttttataatt tgcaactatt ttggcgtatt ctgcttgttt
     1261 ttatcagcga atttttatgc atttcagtca aaagtttgag caggaaaaat gacatttagg
     1321 actttgtaaa attgtattct taaatttgct tgaaaaccta cgtgtacaat tgattttatt
     1381 gaatttttct tacgtttccc gttgtatctt attgtattta actttcatca taaaataatt
     1441 tatttccagg taaaatatcc gacgctctcg tggatccact agttctagct tcaaaagcgc
     1501 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caagatcccc
     1561 gggtaccgag ctcagaaaaa atgacggcac ctaaaaagaa acgcaaagta ccggtagaaa
     1621 aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1681 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1741 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt
     1801 taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta
     1861 ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact
     1921 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1981 acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca tacatattta
     2041 aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     2101 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     2161 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt
     2221 taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc aaaattagac
     2281 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     2341 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     2401 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     2461 ttacacatgg catggacgaa ctatacaaaa gcccacaagc tttggaattc gctagccggc
     2521 catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg
     2581 aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct
     2641 aaaaaattaa ttcaatccaa atctaaacat gagcggtttt ttttctcttt ccgtctccca
     2701 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     2761 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     2821 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     2881 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtctagaat cctatgcttg
     2941 cactttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
     3001 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
     3061 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
     3121 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
     3181 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
     3241 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
     3301 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
     3361 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
     3421 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
     3481 cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc
     3541 tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat
     3601 tcgtttttgg gattcacccc gcggttcgca acaggtttct ttttcaaatt attagcgtta
     3661 taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta
     3721 catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca
     3781 ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt
     3841 ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt
     3901 ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaaca atcgattcca
     3961 cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac
     4021 taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt
     4081 tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg
     4141 cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta
     4201 tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct
     4261 gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa
     4321 gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag
     4381 tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga
     4441 gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt
     4501 cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg
     4561 aattcacgaa acaactgtga acatatctat gagttccctc aactctctca acaactcagt
     4621 gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg
     4681 atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc
     4741 atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg
     4801 ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg
     4861 tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt
     4921 ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca
     4981 cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga
     5041 ttttttcatt agagaatgtc taggcatgca agctagcttc aaaagcgctc tgaagttcct
     5101 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg gattggccaa
     5161 aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc
     5221 cttggagggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     5281 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg
     5341 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5401 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5461 aggcccgcac cgatcgccct tcccaacagt tgcgaaggta agtttaaaca gatccatact
     5521 aactaacttg ttctgacata attttcagct tgaatggcga atggcgcttt gcctggtttc
     5581 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
     5641 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
     5701 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     5761 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     5821 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     5881 taagtttaat taagttgata ctaactaaca aagatctgat taattttcag gacagtcgtt
     5941 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
     6001 tggtgctgcg ttggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
     6061 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
     6121 ttgccactcg ctttaatgat gatttctccc gcgctgtact ggaggctgaa gtccaggtaa
     6181 gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagatg tgcggcgagt
     6241 tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg
     6301 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
     6361 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
     6421 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
     6481 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
     6541 tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
     6601 agacgatggt gcaggatgta agtttaaact attcgttact aactaacttt aaacatttaa
     6661 attttcagat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     6721 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     6781 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     6841 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     6901 tgatcatctg gtcgctgggg aatggtaagt ttaaacagtt gaatactaac taacggagat
     6961 ctttgaaatt ttcagaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     7021 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     7081 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     7141 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgattcttt
     7201 gcgaggtaag tttaaacaga actctactaa ctaacacatt agatcctaat tttcagtacg
     7261 ctcacgcgat gggcaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
     7321 atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
     7381 atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc
     7441 gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgtaagttt
     7501 aaacaataac ctactaacta acgtagataa tttaaatttt caggctgacg gaagcaaaac
     7561 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
     7621 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
     7681 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
     7741 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
     7801 aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcatggcag cagtggaggt
     7861 aagtttaaac aagatcctac taactaactc tacattgatg aattttcaga ctggcggaaa
     7921 acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa
     7981 tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc
     8041 tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca
     8101 cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg
     8161 cctgggtcgg taagtttaaa caaagttgta ctaactaacg aagatcttga taattttcag
     8221 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     8281 atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     8341 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     8401 ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     8461 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     8521 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtagtaag
     8581 tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagtacc ccgtacgtct
     8641 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     8701 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     8761 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     8821 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc caactgagcg
     8881 ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag
     8941 agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc
     9001 gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc
     9061 ctgtgctccc accccctatt tttgttatta tcaaaaaaac ttcttcttaa tttctttgtt
     9121 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     9181 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     9241 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttttacttc tgataaattt
     9301 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     9361 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     9421 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     9481 attaattttc ctgcttttgc tttttggggg tttcccctat tgtttgtcaa gagtttcgag
     9541 gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga
     9601 agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga
     9661 gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata
     9721 actgtttcct actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc
     9781 gcttgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9841 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9901 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9961 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10021 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
    10081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    11161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    11281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    11341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11641 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    12001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    12061 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    12121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12241 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
    12301 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
    12361 atacgctaac aacttggaaa tgaaat
//
