LOCUS       sequence-92540-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Activation module. The plasmid consists of a mutant T7 RNA 
            polymerase (T7 RNAP) activating its own expression via a T7 promoter
            carrying alac operator. Carbenicillin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Payne S, Li B, Cao Y, Schaeffer D, Ryser MD, You L
  TITLE     Temporal control of self-organized pattern formation without 
            morphogen gradients in bacteria.
  JOURNAL   Mol Syst Biol. 2013 Oct 8;9:697. doi: 10.1038/msb.2013.55.
  PUBMED    24104480
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-8"; volume: "9:697. doi"; pages: " 
            10.1038/msb.2013.55"
COMMENT     Sequence Label: pET15bLCFPT7
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..119
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(158..186)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaattaaa gaggagaaag
      121 ctaccgcatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      241 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      301 aacacttgtc actactttga cttggggtgt tcaatgcttt tcaagatacc cagatcatat
      361 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      541 acacaaattg gaatacaact atatttcaca caatgtatac atcactgcag acaaacaaaa
      601 gaatggaatc aaagctcatt tcaaaattag acacaacatt gaagatggaa gcgttcaact
      661 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      721 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
//
