LOCUS       sequence-92543-e        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  replace TgUPRT with DHFR by homologous recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Shen B, Brown KM, Lee TD, Sibley LD
  TITLE     Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii 
            Using CRISPR/CAS9.
  JOURNAL   MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
  PUBMED    24825012
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-05-13"; volume: "5(3). pii: e01114-14. doi"; pages: " 
            10.1128/mBio.01114-14"
COMMENT     Sequence Label: pUPRT::DHFR-D
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtggggcca cgggaggccg ttgttgatgc cgatgcccct cttgggggtc atcgcgacga
       61 ccagacacac cggtttctgc atcttcccag acacgacaac gccccgtaga gcagaaacgc
      121 actactaaag cgaaacttca cccgtccctg ctgcactcag agcagtgctc cgcactgccg
      181 tgtggtaaaa tgaaaaggtt ctacgagaca cgcgtctccg gatcgacaag cgaaggatct
      241 gcacacctgg tctcgatgtc gaacaaagca cggaggagag acggaaagtg cttacatcga
      301 acacggttat caaacccgag aaaaagaaac gaacagaaga aaaaggaaac ctccgcatac
      361 ttttaaagaa tgaagttccc cgattttccc aaaaatggcg tcattttcgc gcacggcagt
      421 cagataacag gtgtagcggc tgcccaccaa cagagacggc gcggccgaca ggacgctact
      481 gggactgcga acagcagcaa gatcggatct tccgcgggcg ggtttgaatg caaggtttcg
      541 tgctgtcgaa aaagagaatg gaacttaatg tccacgtagt tcgcgcatga aaagcgtcct
      601 accactcaaa aaagacaaaa accgcactcg cggcgttgaa tgtgcggggg aacggatggc
      661 tctctttttt ggaaagcgaa tgcaaaaaga caagacgcag acgcatacaa cgttagtccc
      721 ctggcacgag agatagaggt gctgggggcc attgcggtgt cgtggattta ccagtcatgg
      781 acgagatcgt tccggagcct gtctttcctg aaagtctgaa aaaattgcgg gaaagtcacg
      841 catatggaac agcaccgggg cgggacagag ttgtctcgac aacgaatgac acacaggaac
      901 tacgcggggg gtgaaaatcg aatgacatgc tagacttaat taaggatccg gcgcgccccc
      961 gtgaccacgc caaagtagaa aggaattagc ataactcagt caggcagtct ccctgaggac
     1021 acctgcttga tgatttgtga ggacgactca cgggatttac agcc
//
