LOCUS       sequence-92553-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for inserting ubi-CadTS control to Drosophila 
            melanogaster genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Cai D, Chen SC, Prasad M, He L, Wang X, Choesmel-Cadamuro V, Sawyer 
            JK, Danuser G, Montell DJ
  TITLE     Mechanical Feedback through E-Cadherin Promotes Direction Sensing 
            during Collective Cell Migration.
  JOURNAL   Cell. 2014 May 22;157(5):1146-59. doi: 10.1016/j.cell.2014.03.045.
  PUBMED    24855950
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.03"; journalName: "Cell"; date: "2014-05-22- 
            22"; volume: "157"; issue: "5"; pages: "1146-59"
COMMENT     Sequence Label: pUWR-CadTS-C
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(140..391)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(182..203)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     531..550
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgcgcacc ttgaagcgca
       61 tgaactcctt gatgacgtcc tcggaggagg ccatgccatc aaccactttg tacaagaaag
      121 ctgggtgcta ggccgcctac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      181 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagtttgg
      241 actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      301 tctgctggta gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga
      361 agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg tggctgttgt
      421 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      481 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      541 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      601 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc
      661 ccagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg ca
//
