LOCUS       sequence-92555-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette to replace yeast promoters with an inducible promoter 
            containing 3 lexA boxes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-Pmin...
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8..24
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(425..622)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
       61 ccactagtat agaagtatag taatttatgc tgcaaaggtc ctaatgtata aggaaagaag
      121 agaaaagaag aaaacaagag ttttatatac atacagagca catgctctag accctgggtg
      181 tatatacagt tatgctgtga gtatatacag cacctagacc ctgggtgtat atacagttat
      241 gctgtgagta tatacagcac ctagaccctg ggtgtatata cagttatgct gtgagtatat
      301 acagcaccta gggagctcga aaagaaatat agtcttcaag gttatattat acaggtttat
      361 atattattgc ggccgcacta gttcgtttaa acactggatg gcggcgttag tatcgaatcg
      421 acagcagtat agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac
      481 ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat
      541 ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa
      601 caagaatctt tttattgtca gtactgatta ttcctttgcc ctcggacgag tgctggggcg
      661 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
      721 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
      781 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
      841 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
      901 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
      961 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
     1021 tatgcggcca ttgtccgtca ggacattgtt gga
//
