LOCUS       sequence-9256-ex       10215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC185
FEATURES             Location/Qualifiers
     source          1..10215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     443..459
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     444..460
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    475..499
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1963..1987)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2026..2073)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2112..2132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2154..2174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2196..2216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2238..2258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2301..2329)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3077..3097
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(3739..3750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5783..5830)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5869..5889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5911..5931
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5953..5973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5995..6015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6037..6714
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             6757..7431
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     promoter        7960..8064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8065..8133,8134..8925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8283..8302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9096..9684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9585..9604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9838..9855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9972..9993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10008..10013,10014..10031,10032..10038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10046..10062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10051..10073
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10070..10086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10070..10086
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgaggtc gacggtatcg ataagcttga tatcacaagt
      481 ttgtacaaaa aagcaggctc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa
      541 gaatttcggc atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa
      601 ccggtttgaa ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac
      661 aataaattca caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata
      721 catctagaat cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat
      781 tttgatacga cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc
      841 aagataggag cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt
      901 aataatgttc ccaacttaca aaaatgtctc cgtcgacgtt tctgatagtt actagctcga
      961 cataatcaaa atccaacgtt ttctcattca gtaaatccct ttttcgttcc aaataaagag
     1021 gaagaagatg gtcatgacga cgtcgaatgt agtattgatg tgtcttggct gcttctgaaa
     1081 tatttcgatg cggtatgaat ttaatctgta tatacctgtc ggtggtgtcc atccgcttgg
     1141 tgtaggatac ttttcgtgct caggaacatt cggcaccact tctattggca tcaatctttc
     1201 cacatacgac caatcgatag gagcttcttc gaccgttgca agacttctgg ctgtaattgc
     1261 tagtagaaaa attatatgat aataaataac ttacactttt cagggagagc atgtttccat
     1321 ggattttccc aaagtttctc agctttttcg actgaagttg ttgaaaaaga caacctggaa
     1381 actgctcgag gggagaaaat gcggagagaa gaacccagca tcttaataaa attatataat
     1441 tttgaataaa atcaaattaa aaataagatt aattgaaaca caacaataga atgcagaaaa
     1501 ttactcataa gaaataattt attttgaaag aagttgtgtc gatttacgat aaaaactaca
     1561 cgcaaacgag gctccgcgtt tacaccgttt ggcaatggag cgcgcttgct attagatttt
     1621 aggccagctc aacgaaagtt taatttattt ttcttctttt tttccatttt tttgagtaaa
     1681 atatcgaatt ttttgtatta attctgtaaa ttttataatt tgcaactatt ttggcgtatt
     1741 ctgcttgttt ttatcagcga atttttatgc atttcagtca aaagtttgag caggaaaaat
     1801 gacatttagg actttgtaaa attgtattct taaatttgct tgaaaaccta cgtgtacaat
     1861 tgattttatt gaatttttct tacgtttccc gttgtatctt attgtattta actttcatca
     1921 taaaataatt tatttccagg taaaatatcc gacgctctcg tgacccagct ttcttgtaca
     1981 aagtggtgat atcagatcca ctagttctag cttcaaaagc gctctgaagt tcctatactt
     2041 tctagagaat aggaacttcg gaataggaac ttcaagatcc ccgggtaccg agctcagaaa
     2101 aaatgactgc tccaaagaag aagcgtaagg taccggcaga aaaaatgact gctccaaaga
     2161 agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     2221 cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa atgagtaaag
     2281 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     2341 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     2401 ttaaatttat ttgcactact ggaaaactac ctgttccatg ggtaagttta aacatatata
     2461 tactaactaa ccctgattat ttaaattttc agccaacact tgtcactact ttctgttatg
     2521 gtgttcaatg cttctcgaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
     2581 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     2641 agacacgtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtg
     2701 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     2761 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     2821 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt tgtaagttta aacatgattt
     2881 tactaactaa ctaatctgat ttaaattttc agaacttcaa aattagacac aacattgaag
     2941 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     3001 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     3061 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     3121 tggacgaact atacaaaagc ccacaagctt tggaattcgc tagccggcca tacaagtaat
     3181 ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt
     3241 ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt
     3301 caatccaaat ctaaacatga gcggtttttt ttctctttcc gtctcccaat tcgtattccg
     3361 ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg
     3421 acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg
     3481 acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat
     3541 aaaaatatat agaggaggaa ggaaaactag tctagaatcc tatgcttgca ctttgagcca
     3601 atttatccaa gtccttgtaa aaagtattcg aaaattgtta acggataaaa tgtttattat
     3661 aatatcaaaa acaatttgtc agttgaccac tttttgatat atttgacaga aaccgggatg
     3721 aattggctca aagttagggc gcccttctat tacagggttt ctgataacaa accggttatt
     3781 aactcccaac aagggatgat ttcaattcat catgctcaaa ttgacccaaa ttaagttaca
     3841 tgacaaattc atcgcccttt tcaactcttc ttggctcatc atctgttatt ctgttcctat
     3901 tctgtctgca ccctataccc ctttgcatac tctctcgtca ttcctttctt ggatagtgtc
     3961 ttcttctccc cagctctgct acttctatga cttgcgcccg ctgcttttcc gctcgttcct
     4021 ctcctctctg acgtcttctt ctgcttcttt tctccttcct ttttcccttc tcaatcttcc
     4081 tctttttccc atttctgtca atcattcgaa gaagaagaag aagaagaccc tcattcattt
     4141 catttttttt tctgtcggtg tgtgctgctc ggttaagagt gagctctcta ttccacgtct
     4201 tcttcttttt cttcttcgat tcgaatcaat cactccacca aaacgcattc gtttttggga
     4261 ttcaccccgc ggttcgcaac aggtttcttt ttcaaattat tagcgttata aatagaaaaa
     4321 tggtggagtt tcaataaaaa tgataattta caaagtgtat tttgattaca tgtactcaaa
     4381 aaggttgaaa tttttcatac cagttttccg gaaatccatc tgatatcatt atcgtatttt
     4441 tctttttaaa aatgttttca aaaaaaaaca aaatatagct ggttatttgg caccctctaa
     4501 ttaccatttt cctgtcacac cacactcttt tccttcttcc acttctttcc gttttcagcc
     4561 gcttccaacc aaaccgatat gaaagccgag caacaacaat cgattccacc cggctcggcg
     4621 accttcccgt cgcaggtgag actcagaaaa ctagagaaac cgctcaacta actcttgata
     4681 tccgatttca ttcttttctc ttttcttttt gttgaacttt tccatatttc cagatgccac
     4741 ggccaccacc aagcaccgaa caaggaatca caacggaatc ggagcttgcg aagaaagctc
     4801 aaatcactcc gaacgacgtt ttagcacttc cgggaatcac tcaaggtatc ttgctccctt
     4861 tggatttcag tttaaaacat aaatttagga ttcttatgct ccccatctgc gaacatctat
     4921 aacatcgagt tcaccaagtt ccaaatccgt gatctggaca ctgagcaagt gcttttcgag
     4981 atcgccaaac cggagaacga tcaggagaat gatgagtcgc cacaggagtc ggcaagatac
     5041 gtgcgttata gatttgctcc aaactttttg aaactcaaaa cggtcggagc aactgtggaa
     5101 ttcaaagtag gagacatccc aatccatcat ttccgaatga tcgaacgtca cttcttcaaa
     5161 gatcgccttc tgaagtgttt tgactttgaa ttcggattct gtattccgaa ttcacgaaac
     5221 aactgtgaac atatctatga gttccctcaa ctctctcaac aactcagtga gtcattattc
     5281 taaaaagtac aattcaaaag actaatctct ttttcagtgg acgacatgat caacaatcca
     5341 aacgagactc gttctgacag cttctatttc gtcgataaca aactcgtcat gcacaacaaa
     5401 gccgactact catatgatgc ataaatattt aatacaaaaa tgttctggat aattattctg
     5461 tcgaatagaa aaaaaactcc aaatgtgatt aaattccaat aattcctgtc tagtttgttc
     5521 cttccccttc cccttctcat gttcaatgca ttcctaagct tttcagttcc ccccttgttt
     5581 ctatattttt tcgctgtcct gtcacactcg ctaaaaacac taatcacacg gaaatctgtt
     5641 ttcaataaaa actccaactt taactcattt tcaatttcaa ctgaaagatt ttttcattag
     5701 agaatgtcta ggcatgcctg caggtcgact ctagaccgcg gttttggcgc ggatccacta
     5761 gttctagctt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     5821 taggaacttc aagatccccg ggtaccgagc tcagaaaaaa tgactgctcc aaagaagaag
     5881 cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt accggcagaa
     5941 aaaatgactg ctccaaagaa gaagcgtaag gtaccggcag aaaaaatgac tgctccaaag
     6001 aagaagcgta aggtaccggt agacgataag gatccgatgg tggcctcctc cgaggacgtc
     6061 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     6121 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     6181 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
     6241 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     6301 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     6361 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagtt ccgcggcacc
     6421 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     6481 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     6541 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     6601 cagctgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     6661 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat
     6721 ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga cgtcatcaaa
     6781 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
     6841 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     6901 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     6961 aaggcgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     7021 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     7081 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agttccgcgg caccaacttc
     7141 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     7201 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     7261 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcagctg
     7321 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     7381 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta ggaattcgct
     7441 agccggccat acaagtaatc cggatgatcg acgccaacgt cgttgaattt tcaaatttta
     7501 aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt tttttttctt
     7561 gctttctaaa aaattaattc aatccaaatc taaacatgag cggttttttt tctctttccg
     7621 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc
     7681 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga
     7741 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg
     7801 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag
     7861 caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gcccaaaagg cctttttttt
     7921 ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8281 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8581 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9241 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9481 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9721 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9781 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9841 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     9901 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     9961 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10021 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10081 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
    10141 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
    10201 acttggaaat gaaat
//
