LOCUS       sequence-92577-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This self-inactivated retroviral vector has an internal human 
            EF1alpha promoter driving the expression of a bicistronic EGFP ires 
            Puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Rival-Gervier S, Lo MY, Khattak S, Pasceri P, Lorincz MC, Ellis J
  TITLE     Kinetics and epigenetics of retroviral silencing in mouse embryonic 
            stem cells defined by deletion of the D4Z4 element.
  JOURNAL   Mol Ther. 2013 Aug;21(8):1536-50. doi: 10.1038/mt.2013.131. Epub 
            2013 Jun 11.
  PUBMED    23752310
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.131"; journalName: "Mol Ther"; date: "2013-08"; 
            volume: "21"; issue: "8"; pages: "1536-50"
COMMENT     Sequence Label: HSC1-GiP
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    523..539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(547..553,554..571,572..577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    592..613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 acgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
       61 cgcccgaccg aaaggagcgc acgaccccat gcatcgatca tatggataaa ataaaagatt
      121 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
      181 ggagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
      241 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
      301 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
      361 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
      421 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
      481 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
      541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      661 ccagctgcat taatgaatcg
//
