LOCUS       sequence-9258-ex       12245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 12245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC220
FEATURES             Location/Qualifiers
     source          1..12245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4077..4101)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4111..4158)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4234..4254
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4276..4296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4318..4338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4360..4380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4423..4451)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5199..5219
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(6065..6112)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    6187..6207
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(7039..7059)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(7950..7961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9996..10100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10101..10169,10170..10961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10319..10338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11132..11720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11621..11640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11874..11891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12008..12029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12044..12049,12050..12067,12068..12074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12082..12098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12087..12109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12106..12122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12106..12122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcatcaacaa gtttgtacaa aaaagcaggc tgcggcgagg tcggcggtac
      121 gggcgaagaa cttgcgagac aggcggatga ttgaggcttc acactgaaaa tggaaagtta
      181 agctaagaat taagcagatg tttcgaaaaa tattcaaaaa tacaatttga actagacagt
      241 agacctaaga ttgaaaagtt ctgattttca ggtggtctcg aaataaacat tggtacttaa
      301 aacatgtgtt tgcaaatgta tagggtgtca ctaaagtttt gtgtctcaga atgtagtttt
      361 gtgacttcac tgccattggt acgagtcatc tcaccatagt tccaagagca gttggaagct
      421 tttgacagac gttcgagtag agtccgtcaa cagcgtgaga gtgaagagcc tcttggtttt
      481 gagcaaagaa gtgctcgaca gctttacagt tttcacatcc caacataacc ttctctgaca
      541 tggccatttc tacgagagct ggaaattgaa aaattacagg atttcagatg atcgattaac
      601 ttaccatttt ccttcttttc gcatgaatgc atcattgtgc agattcctct tccatcaaat
      661 tgattgtaag tcaagaaaag cacagttgac atatacatat gaacaaagtc attgcatcca
      721 tctgtccagt tggcaaccat cttattgcag gcaactgttt ggatgtcatc agctgttcct
      781 tgctgaaaat ataaaccttt taaaactgct tccaacatac ttttacattt tcttttcaat
      841 acatgcagtg gttattgaaa tttgcaacac aattttctac tgcacttact ctcttatcaa
      901 tgaattcctg aagaagagca tcagcggaaa gtgtgcactc gaagcaagac ataagctcct
      961 ctccgttgct tgtcttcacc attcccatag acttccacat ctcagatgct ggttgtttcg
     1021 gagcttcacg agcagctggc ttggatgtag cagagcagag tcccatgttt gagcacataa
     1081 gttccttttc ggtgaacata tccttcatct cggtcataag atctggaatg acggaggaga
     1141 caaagatgtt gcatgccttg tgggcttttc cggcgctctt gcagacaaag cgttgaaggg
     1201 tagatttgac ctgaaacttg agataatttt accgttcttc ctgcaaaatt taactcactg
     1261 cataggtagt aaattcatcc ccaacgagtt ttccgatctg tgcagttgat gcctgacatt
     1321 cgtcacaaac ttcttgtctc tataaaaatt aattgaaaat taaaaattaa tctcccaaaa
     1381 tgacaaacca taatgttatc attagctacg atggcctctg atttcttcaa gtaaagcatg
     1441 gcaagacgag caagtggtga gaattgagat tcaccgcaca tttggagctt tgagcataca
     1501 gcggaggtgt cagcctgaaa attcagagta ctctctgaaa aaatgtcgta aacctaccaa
     1561 gtatggagca agtttgtagg cgatgaagtc aagtttgtca agagtctttg agcagtcaga
     1621 ttgatgagat gtacctacgc agagcattga aagagaaacc ttcagttgag ccatcttcat
     1681 gcgatcctta gaagcatcaa tgaagttttg aaccatctga aatttgttta ctttatagta
     1741 gtaacattta gttctctgaa tataattact taccgatttg catgattcac atggagatgg
     1801 agcagattgt gctccaagag ccaaagctgc cagcacggcg aagacaaaaa tggatttcat
     1861 tactgcaatt tcaaatttat ttgaaataat ttgtaagaaa ttaatgggaa actatgaaga
     1921 aaactgtgcg aaaaatgaag cggaaaactg aaaacacgtg ttactcggtg ctccttttat
     1981 cacctcttat cagtgtgctc ctccgacgtg tgcctctaat ctgatttatc aggtaattta
     2041 tggtgaagcc cactatacaa cactagagta tgacccggtt tttgattgtt gttaacacga
     2101 ggaaacttca aaaaaataca aaaattccac gaaattgttt atcacaggta attgaaaaag
     2161 tcatcattgt gctcactgaa gcttgaaaaa aaaagagcaa aaattagagc aatgtggtat
     2221 gcgacgttgg tgagagaacg ttcaaggaaa tatgcagata ctggttttta tggaaatttt
     2281 cacagcgtta ttttccggtg cttcataaat ttgaaacagt ctgagtgaca ctttggcact
     2341 cattcagtac tctatgagaa ccaacatact tcgttttacc tttctagaaa tttaaaacat
     2401 ttcgataatt tttctgcaac tgccgaatgg tctagtcgac atctcatctc atttttcaac
     2461 tactataact ttttagactt tttataagaa agccagtaat gtcgtaagct ttacaatttt
     2521 caacttgtgg ataaataatt gtgaactttt tcaaaataac aagcgcaata acttcaaatc
     2581 cacataatga cataccacgt ttcctctact tgttcaatag tctcacactg atactgagaa
     2641 gtcttacgtt tcagaaaggt tggtagagtt gccattaaaa acttcaatgt gctcgaaatg
     2701 ctgataaatt ttggaaagtt ctgtgtgttt ttttactttt aaacggtgtc gaatgaaggt
     2761 tttgtaaaac atcgattgtt tttgccacta ataaggtatg ttttcattgt ccataagtta
     2821 ttgatcaact ttcaattttt ctgatagttc agcaatgttg tagctatttg tcatgaaaat
     2881 gatttattct ttaaacagtt tatcagttat cagcaagcag agaaatgaag aagtttgatg
     2941 ttttcaggtc tagcagttca tctggaatat tttaattaaa atcgagcatt cgaataccca
     3001 aactcacagc ctgactgaat ttgttttggc ttgcaaggac tgcggtcaaa ctgacaaatc
     3061 ttggagcaac agatgacatg atttgttgac actgaaaatt gagatttaag ctttagaaca
     3121 ttaaaaaaaa ctaataaaaa cttacacttg gttggaagaa tgataataat ccagagcaga
     3181 aagtctcagt cagtgagttt tcgaagaatt tctccatggc acttccaaca aggtttgagc
     3241 tcaaatatgt ttccaattcc atcattttcg gacatttttc agcttgaatc tcttggaatg
     3301 tgaaatcacg aagtggggca actgaaaaat agtcggtcga ttattggaca cctcattatt
     3361 acagcaaacc tgaacttgct ggacaacact tgttagctag acaaactcct tgtggagttg
     3421 cctgttcaac tgtcatctga ataacagttg ggagatataa atccaagaaa tcaatacatg
     3481 aatcagtcat gttggctgtg acaatcttgt agcatgcgaa atcacggaca tcatcggaaa
     3541 gtttcttctg aaatcactag aggtgaatca tttaaaaatt tgttttggtg taggtgattg
     3601 aaataaacaa atacacagtt ggattgttgt ttttatgtta cgaaactatt ttaattgttt
     3661 ttgcaataaa cttactctgt tagtgatcat gtctgtttgc atgtcttcaa caacaaactt
     3721 gcattcgaaa caggacatgg cgagagtctg aaaaatggga gtgtattgct tctattcaaa
     3781 ttttgaaaaa taagaaacca catcaaattc cctttttctt gcaaaataac aacgaaatct
     3841 ctttccatct ataaacttat gagcaatatc atcatgttcc aattatttac cgactttgtc
     3901 tcgacaatgt cagtagaagg gtaacatgtg tgaagcaggt ggctgttggg acggcacacg
     3961 attccttttt tagatggtaa agggtcagtt acatctgtca ttttcttttc attactcaac
     4021 tcctacttgg ctcacaactc gtcatttttt atagcgtcat cttacacaat tttattaccc
     4081 agctttcttg tacaaagtgg ttgatgctct gaagttccta tactttctag agaataggaa
     4141 cttcggaata ggaacttcaa gatccccggg tatgtttcga atgatactaa cataacatag
     4201 aacattttca ggagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggca
     4261 gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg cagaaaaaat gactgctcca
     4321 aagaagaagc gtaaggtacc ggcagaaaaa atgactgctc caaagaagaa gcgtaaggta
     4381 ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     4441 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     4501 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     4561 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca
     4621 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa
     4681 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     4741 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca
     4801 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     4861 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     4921 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     4981 gttgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc
     5041 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     5101 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     5161 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     5221 gctgctggga ttacacatgg catggacgaa ctatacaaaa gcccacaagc tttggaattc
     5281 gctagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt
     5341 ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt
     5401 cttgctttct aaaaaattaa ttcaatccaa atctaaacat gagcggtttt ttttctcttt
     5461 ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc
     5521 ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa
     5581 ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg
     5641 aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat
     5701 aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct tgggcccttt cgtctcgcgc
     5761 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     5821 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5881 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     5941 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag
     6001 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6061 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaagatccc
     6121 cgggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagct cattacgcca
     6181 agctatcaac tttgtataga aaagttgatg gcgaagaaac cgagagttac caagctgaat
     6241 acggatcgaa gatcgagagc aaacgagtac gttctcaaat taattacgat aaaattaaca
     6301 ttttcttgaa tttttttagt agttaatacg actcctagca ataaaattga atataatatg
     6361 aaaattagtt agaaaaaaat ataccggtca gaactcaagc ttcatgaaga actgtttcta
     6421 gatagaatgc cccacatttt tcaatatctc agaaatcaca tgcttcatca tcaaaggttt
     6481 attaaacatt gtaaactttc agacgagaac gacagaaagt ttccgagatg aacggaatgt
     6541 ttgatgttct acttaatctt cttccgccgt cacattttaa aacacgactc tccagagttc
     6601 aaatcttgag gtaagttccc caataagatg agatctcttt tagaaaattt tcaaaactct
     6661 acggaagttt acttgtttgt tttcttaatt caaagatttt cattaaaaaa ataaccctcg
     6721 catttcagag aagccacctc ttacataatt cgtcttcaca actttcttat tgaaagttca
     6781 aatagtgaca tagacgcaat aactgtattt ccgcacatct tcaacggaga acgcaagtca
     6841 aataaagatg caattcgtcg tcccatgaag ttgaagcagg gtggcggtgt tgctgctttc
     6901 atctcacgcc acgaacttcc cccacttcaa cttccaaatc ccgttatccc aattttgaag
     6961 cccacttcag ttccagtttg gccccaaacc aatgtttata ttgcttattt ttaaattatt
     7021 gagtttgtat ttgagttgca actttattat acatagttga taattcactg ggctagccgg
     7081 ccatacaagt aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact
     7141 gaatatttgt tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc
     7201 taaaaaatta attcaatcca aatctaaaca tgagcggttt tttttctctt tccgtctccc
     7261 aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat cttctcgctt
     7321 tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa aggatgatgg
     7381 aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg gaggaaggtt
     7441 taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga
     7501 aattaggcga acgatgagaa ttgtcctcgc ttgggccctt tcgtctcgcg cgtttcggtg
     7561 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     7621 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     7681 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7741 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg
     7801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac aacggataaa
     7861 atgtttatta taatatcaaa aacaatttgt cagttgacca ctttttgata tatttgacag
     7921 aaaccgggat gaattggctc aaagttaggg cgcccttcta ttacagggtt tctgataaca
     7981 aaccggttat taactcccaa caagggatga tttcaattca tcatgctcaa attgacccaa
     8041 attaagttac atgacaaatt catcgccctt ttcaactctt cttggctcat catctgttat
     8101 tctgttccta ttctgtctgc accctatacc cctttgcata ctctctcgtc attcctttct
     8161 tggatagtgt cttcttctcc ccagctctgc tacttctatg acttgcgccc gctgcttttc
     8221 cgctcgttcc tctcctctct gacgtcttct tctgcttctt ttctccttcc tttttccctt
     8281 ctcaatcttc ctctttttcc catttctgtc aatcattcga agaagaagaa gaagaagacc
     8341 ctcattcatt tcattttttt ttctgtcggt gtgtgctgct cggttaagag tgagctctct
     8401 attccacgtc ttcttctttt tcttcttcga ttcgaatcaa tcactccacc aaaacgcatt
     8461 cgtttttggg attcaccccg cggttcgcaa caggtttctt tttcaaatta ttagcgttat
     8521 aaatagaaaa atggtggagt ttcaataaaa atgataattt acaaagtgta ttttgattac
     8581 atgtactcaa aaaggttgaa atttttcata ccagttttcc ggaaatccat ctgatatcat
     8641 tatcgtattt ttctttttaa aaatgttttc aaaaaaaaac aaaatatagc tggttatttg
     8701 gcaccctcta attaccattt tcctgtcaca ccacactctt ttccttcttc cacttctttc
     8761 cgttttcagc cgcttccaac caaaccgata tgaaagccga gcaacaacaa tcgattccac
     8821 ccggctcggc gaccttcccg tcgcaggtga gactcagaaa actagagaaa ccgctcaact
     8881 aactcttgat atccgatttc attcttttct cttttctttt tgttgaactt ttccatattt
     8941 ccagatgcca cggccaccac caagcaccga acaaggaatc acaacggaat cggagcttgc
     9001 gaagaaagct caaatcactc cgaacgacgt tttagcactt ccgggaatca ctcaaggtat
     9061 cttgctccct ttggatttca gtttaaaaca taaatttagg attcttatgc tccccatctg
     9121 cgaacatcta taacatcgag ttcaccaagt tccaaatccg tgatctggac actgagcaag
     9181 tgcttttcga gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg ccacaggagt
     9241 cggcaagata cgtgcgttat agatttgctc caaacttttt gaaactcaaa acggtcggag
     9301 caactgtgga attcaaagta ggagacatcc caatccatca tttccgaatg atcgaacgtc
     9361 acttcttcaa agatcgcctt ctgaagtgtt ttgactttga attcggattc tgtattccga
     9421 attcacgaaa caactgtgaa catatctatg agttccctca actctctcaa caactcagtg
     9481 agtcattatt ctaaaaagta caattcaaaa gactaatctc tttttcagtg gacgacatga
     9541 tcaacaatcc aaacgagact cgttctgaca gcttctattt cgtcgataac aaactcgtca
     9601 tgcacaacaa agccgactac tcatatgatg cataaatatt taatacaaaa atgttctgga
     9661 taattattct gtcgaataga aaaaaaactc caaatgtgat taaattccaa taattcctgt
     9721 ctagtttgtt ccttcccctt ccccttctca tgttcaatgc attcctaagc ttttcagttc
     9781 cccccttgtt tctatatttt ttcgctgtcc tgtcacactc gctaaaaaca ctaatcacac
     9841 ggaaatctgt tttcaataaa aactccaact ttaactcatt ttcaatttca actgaaagat
     9901 tttttcatta gagaatgtct aggcatgctc tagagggccc aaaaggcctt ttttttttgg
     9961 cgccgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    10021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    10081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    10141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10321 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10621 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
    11281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11521 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11881 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11941 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    12001 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    12061 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    12121 accatgatta cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt
    12181 aaattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg ctaacaactt
    12241 ggaaa
//
