LOCUS       sequence-92581-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette to replace yeast promoters with an inducible promoter 
            containing 1 lexA box.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-Pmin...
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8..24
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(331..528)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
       61 ccactagtat agaagtatag taatttatgc tgcaaaggtc ctaatgtata aggaaagaag
      121 agaaaagaag aaaacaagag ttttatatac atacagagca catgctctag accctgggtg
      181 tatatacagt tatgctgtga gtatatacag cacctaggga gctcgaaaag aaatatagtc
      241 ttcaaggtta tattatacag gtttatatat tattgcggcc gcactagttc gtttaaacac
      301 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      361 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      421 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      481 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattattcc
      541 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
      601 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
      661 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
      721 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
      781 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
      841 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
      901 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
      961 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     1021 tcatc
//
