LOCUS       sequence-92583-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette to replace yeast promoters with an inducible promoter 
            containing 2 lexA boxes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-Pmin...
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..375
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tgccttgtcc ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga
       61 cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat
      121 acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa
      181 aaattacggc tcctcgctgc ggacctgcga gcagggaaac gctcccctca cagacgcgtt
      241 gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag
      301 gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc
      361 cgaacataaa caaccatggg taaaaagcct gaactcaccg cgacgtctgt cgagaagttt
      421 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
      481 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
      541 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
      601 ccggaagtgc ttgacattgg ggagttcagc gagagcctga cctattgcat ctcccgccgt
      661 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
      721 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
      781 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
      841 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
      901 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
      961 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     1021 attgactgga gcgaggcgat gttcggg
//
