LOCUS       sequence-92585-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette to replace yeast promoters with an inducible promoter 
            containing 2 lexA boxes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-Pmin...
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7..23
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(377..574)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
       61 cactagtata gaagtatagt aatttatgct gcaaaggtcc taatgtataa ggaaagaaga
      121 gaaaagaaga aaacaagagt tttatataca tacagagcac atgctctaga ccctgggtgt
      181 atatacagtt atgctgtgag tatatacagc acctagaccc tgggtgtata tacagttatg
      241 ctgtgagtat atacagcacc tagggagctc gaaaagaaat atagtcttca aggttatatt
      301 atacaggttt atatattatt gcggccgcac tagttcgttt aaacactgga tggcggcgtt
      361 agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta
      421 ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa
      481 tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat acaaatgaca
      541 agttcttgaa aacaagaatc tttttattgt cagtactgat tattcctttg ccctcggacg
      601 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
      661 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
      721 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
      781 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
      841 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
      901 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
      961 aatgaccgct gttatgcggc cattgtccgt caggacattg ttgga
//
