LOCUS       sequence-92586-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from HSC1-GiP where 2 copies of the HS4 insulator core are 
            cloned inside the 3'LTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Rival-Gervier S, Lo MY, Khattak S, Pasceri P, Lorincz MC, Ellis J
  TITLE     Kinetics and epigenetics of retroviral silencing in mouse embryonic 
            stem cells defined by deletion of the D4Z4 element.
  JOURNAL   Mol Ther. 2013 Aug;21(8):1536-50. doi: 10.1038/mt.2013.131. Epub 
            2013 Jun 11.
  PUBMED    23752310
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.131"; journalName: "Mol Ther"; date: "2013-08"; 
            volume: "21"; issue: "8"; pages: "1536-50"
COMMENT     Sequence Label: HSC1-HS4-GiP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     241..257
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     242..258
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    269..302
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
       61 ccgaccgaaa ggagcgcacg accccatgca tcgatcatat ggataaaata aaagatttta
      121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagga
      181 gctctcccat atggtcgacc tgcaggcggc cgcactagtg attcgagctc gagatcgatt
      241 tcgaggtcga cggtatcgcc ggggatctat aacttcgtat aatgtatgct atacgaagtt
      301 atagatcctc tagcgataag cttgatatcg aattcgagtt ggcgcgcctg tcattctaaa
      361 tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga
      421 gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc
      481 cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc
      541 ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg gctttgggag
      601 ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct ttcagcctaa
      661 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
      721 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
      781 cggggatgcg gggggagc
//
